The application of a 96-well plate and steel rivets as a growth surface realizes real-life conditions and at the same time establishes a flexible, simple, fast, and cost-effective assay. All peptides tested in this study demonstrated antibiofilm activity, although these peptides should ...
The influence of myrtenol on CSH of AB was analyzed by MATH assay51. Briefly, AB strains were grown in the absence and presence of myrtenol (50, 100 and 200 μg/mL) and cells were collected, washed with PBS and resuspended in the same. The cell suspensions were adjusted to OD of 0.9...
Furthermore, in vivo mice protection assay was conducted to attest the LAM's anti-pathogenic activity. The current findings elaborated the promising anti-pathogenic and anti-QS activities of LAM. LAM interfered with biofilm formation in P. aeruginosa PAO1 strain. Moreover, swarming motility and ...
Using a microtiter plates-based minimum biofilm inhibitory concentration assay, it was found that doses of these compounds ranging from 2 to 256 mg/mL effectively reduced biofilm formation. The biofilm inhibition assay demonstrated over 80 % reduction of biofilms in all tested species at inhibitory ...
Static biofilm assay The five synthesized DHA compounds (4a,4b,4c,4dand4e) were studied for their anti-biofilm activity, which was evaluated using static biofilm assay. The assay was performed to check the inhibitory activity of the compounds against biofilm formation byB. subtilisandP. aeruginosa...
N-Phenyl-1-naphthylamine (NPN) uptake assay NPN uptake was employed to determine the impact of IPBC on cell wall integrity of both bacteria, as described by27. Bacterial suspensions grown to the exponential phase were centrifuged for 10 min at 4 °C (4000 rpm) and rinsed two times with PB...
Biofilm Formation Assay We then estimated the antibiofilm formation effects of Ag/ZnO nanocomposites combined with LED radiation against S. mutans. These treated planktonic bacteria (107 CFU/mL) were collected and resuspended using the same volume (200 μL) of BHI broth suppleme...
The antibiofilm effect of sub-MIC concentrations of honey samples was analyzed by the microplate biofilm assay. The antimicrobial and antibiofilm activity were screened using by six Gram-positive bacteria; Bacillu...
unrevealing the advantages and limitations of commonly used assays21,22. We were testing the antibiofilm potential ofKlebsiellalytic phage KP34, its recombinant KP34p57 capsule degrading depolymerase (non-bactericidal protein, possessing anti-virulent potential), lytic phage KP15 lacking virion-associated...
Intracellular ATP levels were quantified using an ATP assay kit according to the manufacturer’s instructions (Beyotime Bioengineering Institute, Shanghai, China). Cell membrane integrity The cell membrane permeability of CRKP cells was assessed by confocal laser scanning microscopy (CLSM) according to ...