The scRNA-Seq pipeline involves multiple stages. At first, a live single-cell suspension is created from tissue samples or cell cultures and sorted. Each sample is then combined with a specific antibody and pooled. Next, single cells are lysed to release mRNA and labeled with a unique oligo...
单细胞RNA测序(Single-cell RNA sequencing,scRNA-seq)已成为解剖细胞层次结构的有力工具。 在这项研究中,使用不同AML干细胞、祖细胞和成熟细胞类型的单细胞图谱,通过在AML转录组上的基因表达反卷积,对1000多名AML患者的细胞层次结构进行了描述。这种描述AML异质性的方法使AML基因组和干细胞模型得以整合,从而为理解疾...
The scRNA-Seq pipeline involves multiple stages. At first, a live single-cell suspension is created from tissue samples or cell cultures and sorted. Each sample is then combined with a specific antibody and pooled. Next, single cells are lysed to release mRNA and labeled with a unique oligo...
Single-cell RNA sequencing (scRNA-seq) will enable the classification of AML and guide treatment approaches by profiling patients with different facets of the same disease, stratifying risk, and identifying new potential therapeutic targets at the time of diagnosis or after treatment. ScRNA-seq ...
Integrating Spatial Transcriptome, Single-Cell Sequencing and Histopathology Reveals Bone Marrow Spatial Heterogenous in AML patients(摘要号:2215) 报告时间: 2024年12月7日,星期六,下午5:30至7:30(美国西部时间) 2024年12月8日,...
Table S2. Oligo Sequences and Genotyping Summary per Sample, Related to Figure 3 Table S3. Single-Cell Derived Gene Signatures, Related to Figures 5 and 6 Table S4. Malignant Cell-Type-Specific Genes and Genes Specific to Malignant Monocytes, Related to Figures 6 and 7 Video AbstractVolume...
标题:Single-Cell Transcriptomic Analysis Reveals Distinct Hematopoietic Landscapes and Immune Dysregulation in Der(1;7)(q10;p10) Myelodysplastic Syndromes 类型:poster 作者: 时间:Monday, December 9, 2024: 6:00 PM-8:00 PM ...
Virtually all tumors are genetically heterogeneous, containing mutationally-defined subclonal cell populations that often have distinct phenotypes. Single-cell RNA-sequencing has revealed that a variety of tumors are also transcriptionally heterogeneous,
Integrating Spatial Tranome, Single-Cell Sequencing and Histopathology Reveals Bone Marrow Spatial Heterogenous in AML patients(摘要号:2215) 报告时间: 2024年12月7日,星期六,下午5:30至7:30(美国西部时间) 2024年12月8日,星期日,上午9:30至11:30(北京时间) ...
Single cell RNA sequencing of AML initiating cells reveals RNA-based evolution during disease progressionL. C. Stetson, Dheepa Balasubramanian, Susan Pereira Ribeiro, Tammy Stefan, Kalpana Gupta, Xuan Xu, Slim Fourati, Anne Roe, Zachary Jackson, Robert Schauner, Ashish Sharma, Ban...