Genomic imprinting is an epigenetically regulated process wherein genes are expressed in a parent-of-origin specific manner. Many imprinted genes were initially identified in mice; some of these were subsequently shown not to be imprinted in humans. Such discrepancy reflects developmental, morphological ...
Specifically, "self-DNA" released through exosome secretion en- ters the cytosol of innate immune cells and activates the AIM2 (absent in melanoma 2) inflammasome. This leads to mature IL-1β and IL-18 secretion and induces intestinal mucositis and late-onset diarrhoea. Interestingly, abrogation...
We took two milliliters of blood from the patients and the control group and placed them in an Ethylenediaminetetraacetic acid (EDTA tube), or DNA extraction. Real-time PCR was used to find changes in the genes for CCR5 (rs2227010, rs2734648, rs1799987, rs1799988, rs1...
Extraction of DNA was done using Qiagen DNeasy plant mini kit to generate a DNA template for PCR amplification. 展开 关键词: freeze drying tissue culture cultivars bananas plasmids horticulture genome greenhouses musa leaves DOI: 10.17660/ActaHortic.2010.879.37 ...
of the human genomic gDNA (b) or mtDNA (c).dmtDNA amount of total DNA extractions from THP-1-derived macrophages or bone marrow-derived macrophages (BMDMs) treated for 7 days without or with ethidium bromide (EtBr).e–hELISA analysis of IL-1β secretion from supernatants of THP-1-...
- 《Technology & Health Care Official Journal of the European Society for Engineering & Medicine》 被引量: 13发表: 2011年 DNA extraction from bronchial aspirates for molecular cytology: which method to take? Objective: To date, there are only few systematic reports on the quality of DNA ...
MG132 was an inhibitor of the proteasomal pathway of protein degradation, while chloroquine was an inhibitor of the lysosomal pathway. MG132 and chloroquine were used to treat ACHN and Caki-1 cells for 24h, respectively. Then, the cells were collected for protein extraction and western blots.St...
Cider and/or beer lees has normally either been used as low cost animal feed or been disposed of at great cost. A higher value use for the yeast was therefore sought. This has been developed with the use of environmentally friendly subcritical water extraction. Results have shown that the ex...
Quantitative RT‑PCR analysis The cultured cells were washed twice with Dulbecco's phosphate-buffered saline (Sigma-Aldrich, St Louis, MO, USA) before the isolation of total RNA. RNA extraction was carried out using the RNeasy total RNA extraction kit (QIAGEN, Hilden, Germany) as per the ...
The cytosolic fraction was prepared by centrifugation at 15,000 rcf for 30 min at 4 °C, and the cytosolic DNA was harvested using a G-spin total DNA extraction kit (Intron Biotechnology, Gyeonggi-do, Republic of Korea)34. The copy number of cytochrome c oxidase 1 and 18S rDNA were ...