The results obtained here were highly promising, and demonstrated that the DNA extraction method developed here is compatible with PacBio HiFi sequencing and suitable for de novo whole genome sequencing projects of plants.Background PacBio HiFi long-read DNA sequencing, so-called circular consensus ...
agalactiae and 107 cfu/mL of Staph. aureus with 102 cfu/mL of Strep. agalactiae, respectively. The PCR reactions for Staph. aureus and Strep. agalactiae strains were carried out separately following the PCR conditions described above. Results and Discussion As described in previous studies (...
3. Results and discussion 3.1. Comparing a full demineralization protocol to the Maxwell protocol The manufacturer of the Maxwell instruments launched a semi-automated DNA bone extraction kit (FSC Casework kit, Promega) that instructs the use of 100 mg bone powder and 400 μL demineralization buff...
Please note that I’m using only chromsomes 1-4 as examples, not because they are any more, or less, important than the other chromosomes, but because showing all 22 would not add any benefit to the discussion. The X chromosome has a separate inheritance path and I wrote about thathere....
Results and discussion Initial work with fungal PCR We began investigating fungal ITS primers using ITS1-F as the forward primer situated at the 3' end of SSU and ITS4-B as the reverse primer in the 5' section of LSU [6]. These primers amplify the entire ITS region (Figure 1). The...
We conclude that it is possible to obtain genomic data from resin-entombed organisms, although we discourage aDNA analysis because of the destructive method of extraction protocols and the non-reproducibility of the results.Similar content being viewed by others Recovery and analysis of ancient beetle...
It was observed that all extraction protocols were able to isolate DNA from eight microbial species present in the community standard; however, the overall results suggest that MP kit had better yield and quality as well as higher efficiency in extracting and recovering community DNA from both ...
Results and discussion Initial work with fungal PCR We began investigating fungal ITS primers using ITS1-F as the forward primer situated at the 3' end of SSU and ITS4-B as the reverse primer in the 5' section of LSU [6]. These primers amplify the entire ITS region (Figure 1). The...
Not Availabledoi:10.1016/0379-0738(96)02001-4Manfred N. HochmeisterForensic Science InternationalM.N. Hochmeister, A discussion of `PCR DNA typing of stamps: evaluation of the DNA extraction', Forensic Sci. Int. 83 (1) (1996) 75-79....
We tested nine different DNA extraction methods using three commercial kits (TianLong Stool DNA/RNA Extraction Kit (TS), QIAamp DNA Stool Mini Kit (QS), and QIAamp PowerFecal DNA Kit (QP)) with or without additional bead-beating step using manual or automated methods and compared them in ...