the principles have been shown in Figure 5. For this method, BS-induced DNA degradation may lead to depletion of genomic regions enriched for unmethylated cytosines. Therefore, it is important to assess the amount of DNA degradation under reaction conditions, and how this affects the desired ...
Southern blot hybridization was the original go-to technique of the molecular pathology laboratory for the detection of DNA alterations however one of the disadvantages of this technique is that it requires the extraction of a fairly large amount of DNA (5 to 10 μg) from the fresh sample ...
Evaluation of two DNA extraction methods for the PCR-based detection of eukaryotic enteric pathogens in fecal samples Efficient and easy-to-use DNA extraction and purification methods are critical in implementing PCR-based diagnosis of pathogens. In order to optimize the r... E Menu,C Mary,I To...
Torosin et al. demonstrated that the majority of TADs have been reorganized since the common ancestor ofD. melanogasterandD. triauraria, two species that diverged ~15 million years ago6. In contrast, Renschler
The epicardium, the mesothelial envelope of the vertebrate heart, is the source of multiple cardiac cell lineages during embryonic development and provides signals that are essential to myocardial growth and repair. Here we generate self-organizing human pluripotent stem cell-derived epicardioids that ...
These pools of constructs were transfected, in biological duplicates, into untreated, IFN-α-stimulated or ADAR1-depleted HEK293T cells, as well as into MCF7 and A549 human cancer cell lines and the NIH 3T3 mouse cell line. After RNA extraction, the invariable first arms were reverse ...
Currently, transcriptomes of specific organs, tissues, cells, etc. have been published. Nowadays, there is a wide variety of tools developed to study the transcriptome, from the optimization of the RNA extraction to specific technologies for its analysis, such as DNA microarrays and RNA-seq, to...
Percoll is especially useful as a first step to enrich for cell populations before attempting finer resolution or extraction of nucleic acids. A considerable savings of time and resources may be realized using Percoll as a first step before employing these methods. For biological particles, the ...
In this Review, we present key elements of an iterative DBTL cycle for microbiome engineering, focusing on generalizable approaches, including top-down and bottom-up design processes, synthetic and self-assembled construction methods, and emerging tools to analyse microbiome function. These approaches ...
Adenosine-to-inosine (A-to-I) RNA editing catalyzed by ADAR enzymes occurs in double-stranded RNAs. Despite a compelling need towards predictive understanding of natural and engineered editing events, how the RNA sequence and structure determine the edit