This chapter serves as an introduction to the basic molecular biology behind DNA analysis for those in the seafood industry with limited background in this area, including a review of DNA extraction, polymerase chain reaction (PCR) and gel electrophoresis, as well as a more basic description of...
Material and Methods We developed and validated a DNA typing method for the determination of KEL1/2, JK1/2, FY1/2, FY0, MNS1/2, MNS3/4, DO1/2, CO1/2 and LU1/2 alleles using a melting curve analysis downstream from a fully automated DNA extraction. All assays were validated in ...
derived from evolvedE.coiladenosine deaminase; spCas9n, Cas9 D10A; Rad51DBD, single-strand DNA binding domain; Linkers are also shown.bThe A-to-G base editing efficiency of ABE8e or its fusion constructs at 2 endogenous genomic loci containing multiple As (ABE...
Diversity of the catabolic profiles was calculated using the Shannon index (Shannon and Weaver, 1963) according to the following formula: H ¼ ÀSPi log Pi, where Pi represents the proportion of each particular activity value. DNA extraction and PCR amplification of group- specific 16S rDNA ...
The genus Thielavia is morphologically defined by having non-ostiolate ascomata with a thin peridium composed of textura epidermoidea, and smooth, single-celled, pigmented ascospores with one germ pore. Thielavia is typified with Th. basicola that grows in close association with a hyphomycete ...
Recombinant DNA manipulation was performed as previously described [23]. The plasmid construction and cloning procedures were conducted using the E. coli TOP10 strain. Competent cells of E. coli were prepared using the Competent Cell Preparation Kit (Takara Bio Inc.) and subjected to the respective...
We isolated DNA from the femur muscle of 837 individuals using the Chelex 100 (Bio-Rad Laboratories, Inc.) extraction method (Walsh et al. 1991). We used 13 microsatellite markers that have been specifically developed for M. roeselii: Metroe05, 07, 09, 08, 19, 20, 24, 27 (Kaňuch ...
Kinetic PCR: Real time monitoring of DNA ampli cation reactions We have used the polymerase chain reaction (PCR) to amplify up to 22 kb of the beta-globin gene cluster from human genomic DNA and up to 42 kb from phaga lambda DNA. We have also amplified 91 human genomic inserts of 9-...
Abstract The detection of high molecular organic compounds in ancient remains has turned out to open a new research area with many implications, the most important being the extraction of ancient DNA (aDNA) (to be characterized below) from fossils, subfossil remains, artifacts, traces from biolog...
See Table S2.1 for a summary of the software and packages used. Sampling, DNA extraction, sequencing and processing A total of 183 myna tissue samples in ethanol from India, New Zealand, Australia, South Africa, Hawaii and Fiji between 1975–1989 (Baker and Moeed 19801987; Fleischer et al....