问:如何去除提取的DNA中有RNA污染?答:可按说明书中的方法加入RNase A来消化去除RNA。问:提取的基因组DNA片断长度是多少?答:提取的基因组DNA片段长度一般为30~50KB。核酸的检测分析及图例见英文说明书 Biospin Plant Genomic DNA Extraction Kit Biospin植物基因组DNA提取试剂盒 Cat# BSC13S1 ...
It was applied to the DNA extraction of Gram positive mastitis pathogens directly from milk. The authors claimed that the procedure could be automated with the implementation of a liquid handling system and that high throughput sample processing could be achieved, providing reductions in extraction ...
After the experiment done, students should be able to know how to extract genomic DNA from mammal tissue, and to run an agarosegel. 1.掌握动物基因组DNA提取的操作方法。 2.掌握琼脂糖凝胶电泳的操作的方法 二、实验原理(Experimental Principle) In the procedure described here, the detergents SDS (...
PrincipleQIAamp DNA FFPE Tissue Kit使用成熟的QIAamp MinElute技术,从小体积样本中纯化基因组DNA和线粒体DNA。该试剂盒同时具备硅胶膜选择性结合DNA的特性,以及20 µl到100 µl的灵活洗脱体积。经专门优化的裂解条件,无需过夜温育即可从福尔马林固定、石蜡包埋组织中高效纯化基因组DNA。蛋白酶K消化后在较高的温...
Biospin 细菌基因组DNA提取试剂盒说明书
especially in fungi.Many scientists have been working on selecting a suitable gene which should be used as the standard DNA barcode for fungi.The concept,principle,procedures,advantages and disadvantages of DNA barcode were discussed in the present paper.The prospect for this technology was briefly ...
< DNA Extraction Procedure> Dispense 400-500µL of sample solution to a 2mL blank centrifuge tube. Add 20µL of “Sodium N-Lauryl Sarcosinate Solution, CP” into the tube and mix it with a vortex mixer. Add 500µL of Solution I-B into the tube and mix it with a vortex mixer....
Nucleic acid Extraction Principle The genomic DNA (gDNA) and total RNA are frequently the genetic elements targeted for molecular biology experiments since these are the main sources of genetic information in an organism. Genomic DNA and total RNA extraction have a straightforward methodology, requiring...
但是,如果从革兰氏阳性细菌中提取DNA,机械裂解更加有效(参见"Efficient bacterial DNA extraction")。 Principle DNeasy mericon Food Kit 该试剂盒采用改良的CTAB纯化细胞内核酸。CTAB是非离子去污剂,广泛用于从多种类型的组织样本中高效提纯细胞内核酸。与盐浓度相关,CTAB可能与细胞内核酸结合(低盐浓度);也可与细胞...
To the best of our knowledge, this process represents the first attempt at using zinc powder as a catalyst for the simultaneous oxygen and phosphorus doping of derived-carbon nitride products. The obtained g-POCN was successfully employed in the DNA extraction of vegetal matrix; a procedure that...