Affinity purificationProtein-AProtein-GProtein-LLiquid chromatographyAntibodies are provided in a variety of formats that includes antiserum, hybridoma culture supernatant or ascites. They can all be used successfully in crude form for the detection of target antigens by immunoassay. However, it is ...
Affinity chromatography for purification of antibodies to Neisseria gonorrhoeae and Neisseria meningitidis lipopolysaccharides. Acta Pathol Microbiol Immunol Scand C ... RYBICKI,EP - 《Journal of Phytopathology》 被引量: 11发表: 1986年 Integration of cell harvest with affinity-enhanced purification of monoc...
A technique has been developed for the affinity purification of antibodies recognizing cloned antigens of the malaria parasite Plasmodium falciparum expressed in bacteria. Adsorbents prepared by coupling bacterial lysates to Sepharose were used to isolate monospecific antibodies from human immune sera. Produ...
Affinity Purification of Antibodies Specific for Asn-Linked Glycans Containing α1 → 3 Fucose or β1 → 2 Xylose 来自 ResearchGate 喜欢 0 阅读量: 20 作者:L Faye,V Gomord,AC Fitchette-Lainé,MJ Chrispeels 摘要: Antisera raised against the plant glycoproteins beta-fructosidase and horseradish ...
Purification of anti-paraquat monoclonal antibodies by affinity chromatography on immobilised hapten Affinity chromatography utilizing immobilized antigen was used to purify anti-paraquat antibodies from mouse ascites fluid. A salt wash was required becaus... BJ Horstmann,HA Chase,CN Kenney - 《Journal of...
Ever since the introduction of bacterial derived surface proteins like protein A that demonstrate a natural binding reactivity towards antibodies, affinity chromatography has evolved into a well-established technology for the purification of antibodies a
enable efficient separation of IgGs from other serum constituents. Protein A/G affinity purification of serum-IgGs typically enriches the desired antibody by more than 100-fold. Yet, in the case of anti-cytokine antibodies, the total IgG fraction often contains less than 0.2% of the desired an...
PURIFICATION OF NERVE GROWTH FACTOR ANTIBODIES BY AFFINITY CHROMATOGRAPHY Pure antibodies to nerve growth factor have been isolated from sheep nerve growth factorantiserum by affinity chromatography using 2.5 S nerve growth factor linked to Sepharose 4B by means of cyanogen bromide. The elution of the ...
Affinity precipitation has emerged as a very useful technique for the purification of proteins. Here it has been employed for the purification of anti-bromelain antibodies from rabbit serum. A system has been developed for reversibly binding and thermoprecipitating antibodies. Anti-bromelain antibodies ...
Nontyphoidal Salmonellae (NTS) are a common cause of bacteraemia in children and HIV-infected adults in Sub-Saharan Africa. We have previously shown that antibodies play a key role in both bactericidal and cellular mechanisms of immunity to NTS, but found that high concentrations of antibody to...