As an alternative, we propose a simple small-scale purification protocol without Protein A that uses novel agarose native gel electrophoresis and protein extraction. For large-scale antibody purification, we suggest mixed-mode chromatography that can in part mimic the properties of Protein A resin, ...
Herein we present the first known non-affinity purification method for two S constructs, S_dF_2P and HexaPro, expressed in the mammalian cell line, CHO-DG44. A high-throughput resin screen on the Tecan Freedom EVO200 automated bioprocess workstation led to identification of ion exchange resins...
Method for the purification of LPF-HA A method for producing highly pure LPF-HA (Leucocytosis- promoting Factor Hemagglutinin) from culture media of Bordetella pertussis in a high yield by an affinity chromatography using a denatured ceruloplasmin as a ligand. The LPF-HA obt... S Sakuma,K Sak...
The present invention relates to a novel polypeptide having affinity to a protein containing as a part thereof a CH1-CL domain in which a non-naturally occurring three-dimensional structure is formed, and which can be suitably used for detection, immobilization or removal of these proteins. Specif...
The pure factor of the present invention has a low molecular weight on the order of less than 1000 Daltons. Further, it is non-peptidic and non-lipidic. What is more, it does not have significant cross-reactivity with anti-digoxin antibody. In the final HPLC purification step of the factor...
Here, we used structure- based computational methods to optimize the binding affinity of VHNAC1, a single-domain intracellular antibody (intrabody) from the camelid family that was selected for its specific binding to the nonamyloid component (NAC) of human α-synuclein (α-syn), a natively ...
A method for maintaining intact, non-degraded von Willebrand factor by preventing the action of calcium activated protease(s) responsible for degradation of the factor. The action of the calcium activated protease(s) may be avoided by removing the blood platelet source of the protease(s), by fi...
(J) The affinity of HNF4A on the Tnip3 promoter in Hepa 1-6 cells via chromatin immunoprecipitation (ChIP) assay using an HNF4A antibody or an IgG control, along with RT-PCR experiments with different primer combinations. For (I) and (J), n = 4 independent experiments/group. In all...
expression of CD31 by ASCs. The abundance of CD31 in ASCs was <0.08% of that in HAMVECs, requiring secondary antibody-mediated signal amplification of the directly conjugated anti-CD31 antibody to facilitate its detection by flow cytometry. The binding affinity of antibodies is dependent on the...
A first method to prepare this conjugate consists in the hybridization of a complementary ssON (cON) to the previously synthesized T-ssON. This is typically done by a brief incubation at 37 °C of the two partners, in order to prevent degradation of the antibody (method 1, Fig. 5). ...