Obtained was purified from a mixture containing a host cell protein, as a result, the host cell protein impurities present invention, finally, a protein (eg, an antibody or antibody-like molecules) and by the non-affinity chromatography purification process that combines the HPTFF on research ...
The use of HCIC resin in the capture of non-immunoglobulin proteins comprising at least one Ig-like domain can be scaled up and advantageously combined with other purification and concentration techniques such as ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatogra...
Single-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The linkers were previously derived from self-splicing protein elements called inteins. Two different linkers were generated to solve two distinct separation problems: one for rap...
The pure factor of the present invention has a low molecular weight on the order of less than 1000 Daltons. Further, it is non-peptidic and non-lipidic. What is more, it does not have significant cross-reactivity with anti-digoxin antibody. In the final HPLC purification step of the factor...
A first method to prepare this conjugate consists in the hybridization of a complementary ssON (cON) to the previously synthesized T-ssON. This is typically done by a brief incubation at 37 °C of the two partners, in order to prevent degradation of the antibody (method 1, Fig. 5). ...
expression of CD31 by ASCs. The abundance of CD31 in ASCs was <0.08% of that in HAMVECs, requiring secondary antibody-mediated signal amplification of the directly conjugated anti-CD31 antibody to facilitate its detection by flow cytometry. The binding affinity of antibodies is dependent on the...
CCDC137-overexpressing and control Huh7 cells were lysed and immunoprecipitated with anti-V5 affinity gel. The cell lysates and immunoprecipitants were detected by RT-qPCR. h, i Expressions of FOXM1, JTV1, LASP1 and FLOT2 mRNAs in CCDC137-overexpressing (h) and CCDC137-knockdown (i) cells...
Further genetic manipulations have led to the installation of polyhistidine peptide sequences on the viral capsid using site-directed insertional mutagenesis techniques to allow for the purification of viral particles using affinity chromatography [78]. Strategies such as design-of-experiment methodology im...
A method for maintaining intact, non-degraded von Willebrand factor by preventing the action of calcium activated protease(s) responsible for degradation of the factor. The action of the calcium activated protease(s) may be avoided by removing the blood platelet source of the protease(s), by fi...
The ideal pH in this method is 7.0. At this pH, total charge density is maximal and the dissociated proton and carboxylate molecules of ibuprofen (pKa of 4.9) have a larger affinity with the negatively charged CNTs, hence promoting ibuprofen sorption [89]. Liu et al. [106] evaluated the ...