PurificationVoid-exclusion anion exchange chromatographyCapto MMCMultimodal chromatography resins have been reported to be excellent alternatives to classical absorbents in the purification of antibodies. In this study, void-exclusion anion exchange chromatography (VEAX) and electropositive multimodal ...
Currently, purification of antibodies is mainly carried out using a platform technology composed primarily of Protein A chromatography as a capture step, regardless of the scale. However, Protein A chromatography has a number of drawbacks, which are summarized in this review. As an alternative, we...
Single-step fusion-based affinity purification of proteins with pH-controllable linkers was carried out in a fluidic device. The linkers were previously derived from self-splicing protein elements called inteins. Two different linkers were generated to solve two distinct separation problems: one for rap...
Poly-dC was added by terminal transferase (NEB, Ipswich, MA) to the 3' end of the first strand cDNA (corresponding to the 5' end of the mRNA). The products were purified with QIAquick PCR purification kit again before PCR reactions. The 5'RACE was II High-Fidelity DNA Polymerase (...
As of June 2021, S proteins have exclusively been purified using difficult-to-scale, low-yield methodologies such as affinity and size-exclusion chromatography. Herein we present the first known non-affinity purification method for two S constructs, S_dF_2P and HexaPro, expressed in the ...
Antibodies to Murine Amelogenins: Localization of Enamel Proteins During Tooth Organ Development in vitro During mouse tooth development the secretory ameloblasts differentiate and produce a population of acidic glycoproteins termed 'amelogenins' which function in the formation of the extracellular enamel ...
The purified PCR products were labeled with 32P with a random labeling kit (Stratagene), and unincorporated isotope was purified by a SigmaSpin postreaction purification column (Sigma). Homology Modeling An initial search for templates for homology modeling of Stat5b was carried out using FASTA-...
The excellent performance of the polyVIM material may be attributed to the high recognition ability of VIM for antibodies, as well as the biocompatible and antifouling properties of the porous polymer. This study provides a promising alternative material for the purification of mAbs in downstream ...
Disclosed herein are compositions and methods for the isolation and purification of antibodies from a sample matrix. In particular, the present invention relates to compositions and methods for isolating and purifying antibodies exhibiting weak binding strength and low binding capacity for Protein A resin...
toxins Article Isolation of Anti-Ricin Protective Antibodies Exhibiting High Affinity from Immunized Non-Human Primates Tal Noy-Porat 1, Ronit Rosenfeld 1, Naomi Ariel 1, Eyal Epstein 2, Ron Alcalay 1, Anat Zvi 1, Chanoch Kronman 1, Arie Ordentlich 1 and Ohad Mazor 1,* 1 Department of ...