Methods and compositions are described for analyzing complex protein mixtures using fluorescent activity-based probes. In particular, probes that specifically react with and bind to the active form of one or more target proteins are employed. Fluorescent signals obtained from the labeled active target ...
Mechanism-based probe for the analysis of cathepsin cysteine proteases in living cells. Mechanism-based probes are providing new tools to evaluate the enzymatic activities of protein families in complex mixtures and to assign protein function. The application of these chemical probes for the visualizatio...
The biotinylated activity-based probe was detected with a fluorescent streptavidin Alexa Fluor 647 conjugate (1:10,000) in TBS-T with 1% BSA using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software. The last lane on the membranes is HeLa lysate only. Each ...
Synthesis of bile salt hydrolase (BSH) activity-based probe Azidocholic acid (Thota, Savyasachi, Lukashev, Beletskaya, & Maitra, 2014) (300 mg, 0.7 mmol) was dissolved in anhydrous THF (3.64 mL) and stirred in a dry ice/acetone bath at − 15 °C for 5 min. N-methylmorpholine (...
Based on the mechanism of probe-enzyme interaction, two types of probes that have been used in ABPP are activity-based probes (ABPs) and affinity-based probes (AfBPs). This review highlights the recent advances in the use of ABPs and AfBPs, and summarizes their design strategies (based ...
Activity-based protein profiling: recent advances in probe development and applications. Chem. Bio. Chem. 16, 712–724 (2015). Article CAS PubMed Google Scholar Chen, Z. et al. Functional screening of candidate causal genes for insulin resistance in human preadipocytes and adipocytes. Circ. ...
The charge densities associated with the adsorption/desorption of probe species (e.g., H, CO, and Cu) obtained from cyclic voltammetry are commonly used to estimate the ECSAs of various Pt-based electrocatalysts [60–62]. The specific activity allows to acquire relevant information on the size...
One such approach, valid for 2-D equilibria, is based on a generalization of the broad class of isotropic CS models (Schindler 1972). These models use the solution of the Vlasov equation in the form of an arbitrary function of two integrals of motion, the total energy E_{\alpha} of par...
More advanced functionality, like sending HTML-based e-mail, is available through this component. You can find more details regarding CDONTS in Knowledge Base articleQ186204. Scheduling the Tool Using the Windows Task Scheduler, you can set the Web monitoring tool to run periodically. You can run...
After recovery, neural activity was recorded using Neuropixels 1.0 (n = 5) and 2.0 (n = 3, among which two had four shanks) probes implanted in left primary visual cortex (2.5 mm lateral, 3.5 mm posterior from Bregma, one probe per animal) and in the underlying hippocampal...