ACTIVITY BASED PROBE AND USES THEREOF FOR IMAGINGProvided is a compound including at least one carrier moiety associated with a plurality of CT imaging moieties, and with at least one enzyme interacting moiety as well as uses thereof in diagnosis.Galia BLUM...
Activity-Based Imaging Probes of the Proteasome 来自 Springer 喜欢 0 阅读量: 24 作者:Kimberly,Cornish,Carmony,Kyung,Bo,Kim 摘要: Over the years, the proteasome has been extensively investigated due to its crucial roles in many important signaling pathways and its implications in diseases. Two ...
The biotinylated activity-based probe was detected with a fluorescent streptavidin Alexa Fluor 647 conjugate (1:10,000) in TBS-T with 1% BSA using an Azure Biosystems Sapphire Biomolecular Imager and Azure Spot Analysis Software. The last lane on the membranes is HeLa lysate only. Each ...
selectivity based on the docking model with in cellulo evidence, we analyzed images of mitotic cells treated with QPD-OTf. Eg5 associates with the mitotic spindle47,48, hence, Eg5 hydrolysis should result in fluorescence at the mitotic spindle. Imaging of mitotic HeLa-GFP-Tubulin cells treated...
Non-invasive imaging of cysteine cathepsin activity in solid tumors using a 64Cu-labeled activity-based probe. PLoS One. 2011;6:e28029. Article PubMed Central CAS PubMed Google Scholar Kosinska MK, Liebisch G, Lochnit G, Wilhelm J, Klein H, Kaesser U, et al. A lipidomic study of ...
In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achieve molecularly sensitive imaging contrast in optical projection tomography by means of targeted and activatable...
IA-alkyne is included as a general, cysteine-reactive probe counterpart for benchmarking PACCE. Data shown are derived from HEK293T cells. B Proteins containing RS-Cys sites (aggregate from PACCE in situ analysis of DM93 and HEK293T cells) were compared with proteins detected by RNA-based ...
Hitherto, the development of an activity-based fluorescent probe for the precise imaging of β-gal in cabbage roots continues to present challenges. Here, we introduce BC-βgal, an activatable fluorescence probe that utilizes intramolecular charge transfer (ICT) technology. To address possible ...
Finally, cytotoxicity of the imaging probe is an important consideration. Metal complexes have been widely implicated as initiators of different apoptotic pathways [[32], [33], [34]]. Several cellular processes and changes occur during initiation of cell death including caspase activation [35,36]...
A novel scheme for scanning electrochemical microscopy (SECM) assay of DNA based on hairpin probe and enzymatic amplification biosensor was described. In t... Z Zhang,J Zhou,A Tang,... - 《Biosensors & Bioelectronics》 被引量: 40发表: 2010年 Constant-distance mode SECM as a tool to visual...