Fluorescent probeActivity-based sensingPeroxynitriteInflammationTumorPeroxynitrite (ONOO ), one of the reactive oxygen and nitrogen species (RONS), is famous for its potent oxidation and nitration capabilities and plays crucial roles in various pathological conditions. Given the similar chemical properties ...
We describe a fluorescent DNA-based probe technology, NOckout, that can be targeted either to the plasma membrane or the TGN, where it can quantitatively map the activities of endogenous NOS3 at these locations in live cells. We found that, although NOS3 at the Golgi is tenfold less ...
The presence of the cell wall poses challenges for fluorescent probes to penetrate and selectively interact with β-gal in plant cells. Hitherto, the development of an activity-based fluorescent probe for the precise imaging of β-gal in cabbage roots continues to present challenges. Here, we ...
thus acting as an activity-based fluorescent reporter49. While this approach has been very productive in image hydrolytic enzymes, such as protease and glycosidase, with a broad tolerance for the leaving group, there are no examples reported for motor proteins. The discovery of a fluorogenic substr...
To this end, we herein sought to assess a cathepsin activity-based probe (ABP), GB123, in vitro and in vivo. Methods Protein levels and activity of cathepsins B and S were monitored by immunoblot analysis and GB123 labeling in cultured primary chondrocytes and conditioned media, following ...
IA-alkyne is included as a general, cysteine-reactive probe counterpart for benchmarking PACCE. Data shown are derived from HEK293T cells. B Proteins containing RS-Cys sites (aggregate from PACCE in situ analysis of DM93 and HEK293T cells) were compared with proteins detected by RNA-based ...
a fluorescent probe for butyrylcholinesterase activity in human serum based 系统标签: fluorophore serum probe fluorescent binding albumin Registeredcharitynumber:207890rsc.li/chemcommShowcasingresearchfromProfessorMinSuHan’slaboratory,DepartmentChemistry,GwangjuInstituteTechnology(GIST),Gwangju,RepublicSoyeonYoo.flu...
We tried to attach fluorescent probes with the Y174C mutant, but the labeling efficiency was poor. Other cysteine substitution mutants will be explored in future related mechanistic studies. Cysteine substitution mutants of MtTOP1 could potentially be utilized for development of enzyme based HTS ...
In this work, one activity-based non-peptide ratiometric fluorescent probe DCDF was designed with pentafluoropropionic anhydride as identification group. To our knowledge, this is the first probe capable of detecting NE in ratio. After the addition of the NE, the emission spectrum of DCDF has ...
Assembly fluorescent probe-based detection of gamma-glutamyl transferase activity in tumor Detection of 纬-glutamyltransferase (GGT) activity in cancer cells is crucial for understanding tumor progression and metabolic dysregulation. However, the accurate detection of GGT remains challenging due to its ...