activity‐based probesactivity‐based protein profilingalkyl linkeranalytical platformscapillary electrophoresis platformenzyme classesgel‐based platformsmass spectrometry platformsreactive groupreporter tagThis chapter deals with various aspects of activity‐based protein profiling (ABPP) including the general ...
The IA-alkyne probe has been used to map cysteine-reactivity of endogenously-derived lipid aldehydes such as hydroxynonenal and S-nitrosoglutathione [41, 42•]. Wang et al. ABPP with reactivity-based probes or reactive chemical scaffolds to develop small-molecule inhibitors against ligandable ...
271Citations 18Altmetric Metrics Key Points Activity-based protein profiling (ABPP) facilitates the discovery of deregulated enzymes in cancer. Competitive ABPP yields selective inhibitors for functional characterization of cancer enzymes. ABPP can be integrated with metabolomics to map deregulated enzymatic ...
Competitive activity-based protein profiling (ABPP) would aid the development and validation of new selective inhibitors. Here, we present a broad-spectrum activity-based probe that reports on several aldehyde dehydrogenases. This probe was used in a competitive ABPP protocol against three ALDH ...
Here, we showcase the toolbox frequently used for the analysis of DUB activity and illustrate its application by profiling pathogen-derived proteases toward Ub and Ubls. 2. Activity-based probes 2.1. Probes based on a monoUb or Ubl recognition element The first activity-based DUB probe was ba...
Activity-BasedProteinProfiling;ABPP;ClickChemistry;MassSpectrometry;MudPIT;Activity- BasedProbes;Biotin;Alkyne;Azide INTRODUCTION Thefieldofproteomicsaimstocharacterizeandassignfunctiontothetensofthousandsof eukaryoticandprokaryoticproteinsannotatedbygenomesequencingefforts.Incontrastto ...
Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse—small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assay, we...
To this end, we herein sought to assess a cathepsin activity-based probe (ABP), GB123, in vitro and in vivo. Methods Protein levels and activity of cathepsins B and S were monitored by immunoblot analysis and GB123 labeling in cultured primary chondrocytes and conditioned media, following ...
An activity-based fluorogenic probe for sensitive and selective monoamine oxidase-B detection. Shaobo Long,Lin Chen,Yumi Xiang. Chemical Communications . 2012Long, S. B. et al. An activity-based fluorogenic probe for sensitive and selective monoamine oxidase-B detection. Chem. Commun. 48, 7164-...
Analytical platforms for activity-based protein profiling - Exploiting the versatility of chemistry for functional proteomics The field of proteomics aims to develop and apply technologies for the characterization of protein function on a global scale. Toward this end, synthetic c... Sieber, SA,...