Hence, we attempted to provide the first comprehensive profiling of active serine hydrolases (SHs) present in rice bran proteome by activity-based protein profiling (ABPP) strategy. The active site-directed fluorophosphonate probe (rhodamine and biotin-conjugated) was used for the detection and ...
Activity-based protein profiling (ABPP) facilitates the discovery of deregulated enzymes in cancer. Competitive ABPP yields selective inhibitors for functional characterization of cancer enzymes. ABPP can be integrated with metabolomics to map deregulated enzymatic pathways in cancer. ...
While many activity-based probes have been developed by the chemical biology field over the last decade, there are still many different enzyme classes and more importantly a diversity of protein functionalities that are not detectable ABPP with reactivity-based probes to map the proteome-wide targets...
ABPP Methodology: Introduction and Overview, by Matthew B. Nodwell und Stephan A. Sieber Activity-Based Protein Profiling for Natural Product Target Discovery, by Joanna Krysiak und Rolf Breinbauer Photoaffinity Labeling in Activity-Based Protein Profiling, by Paul P. Geurink, Laurette M. P...
Competitive activity-based protein profiling (ABPP) would aid the development and validation of new selective inhibitors. Here, we present a broad-spectrum activity-based probe that reports on several aldehyde dehydrogenases. This probe was used in a competitive ABPP protocol against three ALDH ...
However, standard protein or transcript abundances are blind to the activity status of the measured enzymes, regulated, for example, by active-site amino acid mutations or post-translational protein modifications. Current methods for activity-based proteome profiling (ABPP), which combine mass ...
Activity-BasedProteinProfiling;ABPP;ClickChemistry;MassSpectrometry;MudPIT;Activity- BasedProbes;Biotin;Alkyne;Azide INTRODUCTION Thefieldofproteomicsaimstocharacterizeandassignfunctiontothetensofthousandsof eukaryoticandprokaryoticproteinsannotatedbygenomesequencingefforts.Incontrastto ...
Activity-based protein profiling (ABPP) is a technique that offers direct insight into changes in catalytic activity of enzyme classes in complex proteomes (18, 19) and can be used to functionally annotate the enzyme function of proteins (20). ABPP has been successfully applied to a number of...
Activity-based protein profiling (ABPP) is a chemical proteomic method for functional interrogation of enzymes within complex proteomes. This Unit presents a protocol for in vitro and in vivo labeling using ABPP and Click Chemistry (CC)-ABPP probes for in-depth profiling using the Multi-dimensional...
Activity-based protein profiling (ABPP) has been used extensively to discover and optimize selective inhibitors of enzymes. Here, we show that ABPP can also be implemented to identify the converse—small-molecule enzyme activators. Using a kinetically controlled, fluorescence polarization-ABPP assay, we...