Activity-Based Probes (ABPs) have been used to dissect the biochemical/structural properties and cellular functions of deubiquitinases. However, their utility in studying cysteine-based E3 ubiquitin ligases has so far been limited. In this study, we evaluate the use of Ubiquitin-ABPs (Ub-VME ...
Activity-based probes (ABPs) are small molecules that exclusively form covalent bonds with catalytically active enzymes. In the last decade, they have especially been used in functional proteomics studies of proteases. Here, we present phosphoramidate peptides as a novel type of ABP for serine ...
Based on the mechanism of probe-enzyme interaction, two types of probes that have been used in ABPP are activity-based probes (ABPs) and affinity-based probes (AfBPs). This review highlights the recent advances in the use of ABPs and AfBPs, and summarizes their design strategies (based ...
Activity-based probes (ABPs) are reactive small molecules that covalently bind to active enzymes. When tagged with a fluorophore, ABPs serve as powerful tools to investigate enzymatic activity across a wide variety of applications. In this chapter, we provide detailed methods for using fluorescent ...
Here, we demonstrate that activity-based probes (ABPs) targeting cysteine cathepsins can be used in murine models of atherosclerosis to non-invasively image activated macrophage populations using both optical and PET/CT methods. The probes can also be used to topically label human carotid plaques ...
Activity-based proteomics, often referred to as activity-based protein profiling (ABPP) has become a promising option to circumvent this limitation. In summary, active-site directed chemical structures linked to a reporter tag, so-called activity-based probes (ABPs), are employed to covalently ...
Chemoproteomics has the unique ability to identify enzymatic activity within complex biological mixtures and relies on the use of activity-based probes (ABPs) to target specific enzymes (Niphakis & Cravatt, 2014). An ABP is a substrate analog that typically includes a targeting group that is spec...
While a number of reactive functional groups have been applied to activity-based probes (ABPs) that target diverse families of proteases, there remains a... Z Yang,Marko Fonovi,Steven H.L. Verhelst,... - 《Bioorg Med Chem》 被引量: 23发表: 2009年 'Angioprevention': angiogenesis is a ...
Activity was analyzed using subunit-specific activity-based probes (ABPs) and native gel analysis. In COPD, expression of the immunoproteasome subunits LMP2, LMP7 and MECL1 was increased and activity was also slightly albeit non-significantly upregulated. We are currently analyzing local immune ...
26,27 Therefore, to comprehensively evaluate the genuine target of microcolin H, we designed and synthesised a set of activity-based probes 1–5 according to the structure-activity relationship of analogues A1-A5 (Fig. 4a). Notably, the capacity of probe 4 to inhibit cancer cell proliferation...