Our probe also unveiled differential rewiring of CRL networks across distinct primary cell activation pathways. Thus, conformation-specific probes can permit nonenzymatic activity-based profiling across a system of numerous multiprotein complexes, which in the case of neddylated CRLs reveals widespread ...
The IA-alkyne probe has been used to map cysteine-reactivity of endogenously-derived lipid aldehydes such as hydroxynonenal and S-nitrosoglutathione [41, 42•]. Wang et al. ABPP with reactivity-based probes or reactive chemical scaffolds to develop small-molecule inhibitors against ligandable ...
If this sulfonate displayed a high reactivity with generic nucleophiles, then the probe’s effective concentration in thiol-treated proteome reactions should be greatly reduced, resulting in a significant decrease in the signal intensity of specifically labeled proteins. However, none of the tested ...
Metrics Key Points Activity-based protein profiling (ABPP) facilitates the discovery of deregulated enzymes in cancer. Competitive ABPP yields selective inhibitors for functional characterization of cancer enzymes. ABPP can be integrated with metabolomics to map deregulated enzymatic pathways in cancer. ...
Competitive activity-based protein profiling (ABPP) would aid the development and validation of new selective inhibitors. Here, we present a broad-spectrum activity-based probe that reports on several aldehyde dehydrogenases. This probe was used in a competitive ABPP protocol against three ALDH ...
activity‐based protein profilingdeubiquitinating enzymesfluorescent probessolid‐phase synthesisubiquitinEpitope-tagged active-site-directed probes are widely used to visualize the activity of deubiquitinases (DUBs) in cell extracts, to investigate the specificity and potency of small-molecule DUB inhibitors,...
The ubiquitin-proteasome system (UPS) is essential for Plasmodium falciparum survival and represents a potential target for antimalarial therapies. We utilised a ubiquitin- activity based probe (Ub-Dha) to capture active components of the ubiquitin conjugating machinery during a...
待分类 系统标签: argininephosphorylationproteinoxidativeywleactivity ArticleActivity-BasedProfilingRevealsaRegulatoryLinkbetweenOxidativeStressandProteinArgininePhosphorylationGraphicalAbstractHighlightsdGenerationofSO3-amidinebasedproteinargininephosphataseinhibitorsdDevelopmentofanactivity-basedaffinityprobe(ABAP)forarginine...
of probe labeling (Figure 1: box). For standard ABPP using biotinylated probes, follow Basic Protocol 1; for profiling enzyme activities in living cells or mice using alkyne probes, use Alternate Protocol 1. Both of these protocols will generate labeled proteomic samples for enrichment, digestion ...
Here, we showcase the toolbox frequently used for the analysis of DUB activity and illustrate its application by profiling pathogen-derived proteases toward Ub and Ubls. 2. Activity-based probes 2.1. Probes based on a monoUb or Ubl recognition element The first activity-based DUB probe was ba...