Several actin-binding proteins (ABPs) phase separate to form condensates capable of curating the actin network shapes. Here, we use computational modeling to understand the principles of actin network organization within VASP condensate droplets. Our sim
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Our study shows that LASP-1, an actin-binding protein, is highly expressed in podocytes. Electron microscopy studies demonstrate that LASP-1 is found at the slit membrane suggesting a role in anchoring slit membrane components to the actin cytoskeleton. Live cell imaging experiments with transfected...
A network of actin-binding proteins, some of which can undergo liquid–liquid phase separation, controls filament bundling. However, it remains unclear how these liquid-like condensates contribute to filament bundling. Here we show that the processive actin polymerase and bundling protein VASP forms ...
The focal adhesion protein vinculin is an actin-binding protein involved in the mechanical coupling between the actin cytoskeleton and the extracellular matrix. An autoinhibitory interaction between the N-terminal head (Vh) and the C-terminal tail (Vt) of vinculin masks an actin filament side-bindi...
The localization of GFP-MYA2-head6IQ suggests a weak in vivo F-actin-binding The lower quality of filamentous signals induced by the GFP-MYA2-head6IQ, compared with other F-actin markers, is not fully explained by artifacts due to overexpression or protein degradation. The expression times ...
These results implicate the RIL PDZ-LIM protein as a regulator of actin stress fiber turnover. 展开 关键词: Actin Cytoskeleton Dynamics Nonmuscle Live cell imaging DOI: 10.1016/j.yexcr.2003.09.004 被引量: 124 年份: 2004 收藏 引用 批量引用 报错 分享 ...
Pollen tube growth is dependent on the actin cytoskeleton; its organization and regulation have been examined extensively by various approaches, including fluorescent protein labeled actin-binding proteins in live cell studies. Using the previously described GFP-NtADF1 and GFP-LlADF1, and a new actin...
Nunoue, K., Ohashi, K., Okano, I. & Mizuno, K. LIMK-1 and LIMK-2, two members of a LIM motif-containing protein kinase family.Oncogene11, 701–710 (1995). CASPubMedGoogle Scholar Ridley, A. J. & Hall, A. The small GTP-binding protein rho regulates the assembly of focal adhesi...
microtubules get enriched in detyrosinated tubulin. During fragmentation, the MPS is lost, but βII-spectrin and F-actin are still present. When fragments become round beads-like structures, βII-spectrin and F-actin are almost absent, while they continue to be positive to markers of microtubu...