图4. rAAV8-D377Y-mPCSK9诱导动脉粥样硬化,主动脉弓动脉粥样硬化存在剂量依赖性(高剂量组:5.0×10^11vg;中等剂量组:1.0×10^11vg;低剂量组:2.0×10^10vg,Bjørklund MM, et al.Circulation Research. 2014)表1. AAV-PCSK9与Ldlr KO小鼠、ApoE KO小鼠构建AS模型的对比(Emini Veseli B, et al. Eur ...
图6.AAV-PCSK9与Ldlr KO小鼠、ApoE KO小鼠构建AS模型的对比（Emini Veseli B, et al. Eur J Phar...
为了实现长期的高胆固醇血症,研究者将不同剂量的重组腺相关病毒载体(人源及小鼠PCSK9DY载体设计图见图2)注射至C57BL/6NTac小鼠中,然后进行高脂饮食,12周后,小鼠出现动脉粥样硬化,主要表现在主动脉弓或主动脉根(图3)。 图2. PCSK9DY载体示意图 图3. AAV-PCSK9DY诱导小鼠动脉粥样硬化 (Bjorklund,M .M ., ...
为了实现长期的高胆固醇血症,研究者将不同剂量的重组腺相关病毒载体(人源及小鼠PCSK9DY载体设计图见图2)注射至C57BL/6NTac小鼠中,然后进行高脂饮食,12周后,小鼠出现动脉粥样硬化,主要表现在主动脉弓或主动脉根(图3)。 图2. PCSK9DY载体示意图 图3. AAV-PCSK9DY诱导小鼠动脉粥样硬化 (Bjorklund,M .M ., ...
2、本发明的第二目的在于提供一种pcsk9基因重组aav载体,其能够高效靶向转导肝脏组织,并使pcsk9基因长期稳定表达。 3、本发明的第三目的在于提供一种高脂血症动物模型的构建方法可自发形成显著的高胆固醇血症和高甘油三酯血症表型。 4、本发明的第四目的在于提供上述pcsk9基因重组aav载体和构建方法在药物筛选或制备用...
An adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding anti-PCSK9 antibody. In desired embodiments, the subject is a human.Wilson, James M.Jacobs, FrankSomanthan, Suryanrayan
C57 mice were injected with AAV9-PCSK9 or AAV9-luciferase (control) and high-fat diet was initiated. A week later, partial ligation was performed. Compared to the control, AAV-PCSK9 led to elevated serum PCSK9, hypercholesterolemia, and rapid atherosclerosis development within 3 weeks as ...
AAV-ANTI PCSK9 ANTIBODY CONSTRUCTS AND USES THEREOFCompositions and methods are provided for lowering cholesterol in a subject are provided. An adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding anti-PCSK9 antibody. In desired embodiments, ...
AAV-ANTI PCSK9 ANTIBODY CONSTRUCTS AND USES THEREOFCompositions and methods are provided for lowering cholesterol in a subject are provided. An adeno-associated viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding anti-PCSK9 antibody. In desired embodiments, ...
AAV-PCSK9DYmouse modelAtomic force microscopyCortical stiffnessEndothelial dysfunctionInvestigating atherosclerosis and endothelial dysfunction has mainly become established in genetically modified ApoE / or LDL-R / mice transgenic models. A new AAV-PCSK9DY DY mouse model with no genetic modification has ...