对于 scRNA-seq,跨越 7 个发育时间点(12、13、14、15、18、19 和 20 个 PCW)的 12 个个体胚胎的整个胰腺被解离,活分选的单细胞与结合到普遍存在的表面标志物 b2M 和 CD298,它们由发育中的人类胰腺表达。使用 10x Chromium protocol对细胞进行测序,并在严格过滤后保留 24,080 个高质量细胞用于下游分析(12pc...
对于 scRNA-seq,跨越 7 个发育时间点(12、13、14、15、18、19 和 20 个 PCW)的 12 个个体胚胎的整个胰腺被解离,活分选的单细胞与结合到普遍存在的表面标志物 b2M 和 CD298,它们由发育中的人类胰腺表达。使用 10x Chromium protocol对细胞进行测序,并在严格过滤后保留 24,080 个高质量细胞用于下游分析(12pc...
Spatial transcriptomics, commercialized as Visium Spatial Gene Expression, is a molecular profiling method that enables scientists to map whole transcriptome gene activity across an entire tissue section alongside H&E morphological imaging.The next evolution of this platform—powered by Visium CytAssist, ...
自2020年10x genomics率先推出冰冻切片的空间转录组,不久之后10x 就发布了空间转录组在FFPE组织切片的protocol和分析软件,冰冻切片的运用早已取得了极大的进展,但是FFPE由于其仅有空间切片,缺少匹配的单细胞数据而限制了其广泛的运用,时至今日,全球首先运用FFPE空间转录组的文章Tertiary lymphoid structures generate and pr...
Single-cell transcriptomics of the mouse kidney reveals potential cellular targets of kidney disease. Science. 2018;360(6390):758-763. 空间转录组: 1.Stahl PL, Salmén F, Vickovic S, et al. Visualization and analysis of gene expression in tissue sections by spatial transcriptomics. Science. ...
虽然大多数细胞类型是从所有位置和protocol中分离出来的,但细胞类型比例存在明显差异。正如预期的那样,外膜成纤维细胞 (Fibro-adv) 在气道样本中富集,而肺泡成纤维细胞 (Fibro-alv) 被耗尽。We further observed a rare cell type recovered only from the single cells, but not from single nuclei. 这种罕见的...
The spatial “where” of gene expression has lagged behind the cellular “what” provided by single cell because spatial transcriptomics requires a different—and more challenging—approach. We’re immensely proud to be one of the driving forces in spatial transcriptomics technologies, but some ...
Now, I’d like to share more about our spatial platforms. Through our internal investments and our acquisitions of Spatial Transcriptomics, ReadCoor and CartaNA AB, 10x has been leading the way in spatial for a decade. We have built incredibly powerful technologies, developed foundational intellectua...
Single-Cell Transcriptomics: For gene expression analysis at the RNA level. Single-Cell CNV-Seq: For examining copy number variations (CNVs) at the DNA level. Feature Barcode Protein Detection: For profiling surface proteins using feature barcoding. ...
虽然大多数细胞类型是从所有位置和protocol中分离出来的,但细胞类型比例存在明显差异。正如预期的那样,外膜成纤维细胞 (Fibro-adv) 在气道样本中富集,而肺泡成纤维细胞 (Fibro-alv) 被耗尽。We further observed a rare cell type recovered only from the single cells, but not from single nuclei. 这种罕见的...