该论文的通讯作者Joakim Lundeberg也是瑞典Spatial Transcriptomics公司的联合创始人之一,2018年底10X Genomics宣布收购Spatial Transcriptomics,并于2019年发布Visium空间基因表达解决方案(Visium Spatial Gene Expression Solution)。 02、10X Genomics Visium空间转录组技术 1、技术原理 将冰冻组织切片放置在10X Genomics Visium芯...
为什么选择10x Genomics的空间转录组学? 空间基因表达(或空间转录组学)是指映射到组织切片中的特定位置的全转录组或靶向基因表达的定量读数,也是一种在原生组织背景下了解细胞组成和活性的强大方法。 空间转录组学可以通过基于新一代测序的方法实现,这种方法在全转录组水平上将mRNA映射到组织中,然后进行体外测序。我们的...
Spatial transcriptomics combines high-plex quantification with the spatial resolution of immunohistochemistry. This technology classifies tissue based on total mRNA within the morphological context of FFPE or frozen tissues. Spatial expression mapping is a powerful tool for examining cellular interactions, tiss...
Spatial transcriptomics technologies are assessed by key metrics, such as resolution of the spatial biology, gene plexity (the number of genes detected), and gene sensitivity (the linear dynamic range of accurate transcript detection). 10x Genomics spatial tools are optimized to provide robust insight...
Demonstrated the Visium HD assay has superior spatial fidelity compared to other commercially available sequencing-based spatial transcriptomics platforms. bioRxiv, Dec 2024 Ren P et al. Read on bioRxiv Characterization of immune cell populations in the tumor microenvironment of colorectal cancer using hi...
Spatial transcriptomics was performed by Visium CytAssist (10X-Genomics). I148M-protein upregulation in hepatoma cells fostered lipid accumulation, thus increasing PGC1a to clear fat. Both I148M-overexpressed models showed lower OXPHOS capacity and ATP production alongside higher release of ROS and ...
Spatial transcriptomics produces high-dimensional gene expression measurements with spatial context. Obtaining a biologically meaningful low-dimensional representation of such data is crucial for effective interpretation and downstream analysis. Here, we
et al. High-definition spatial transcriptomics for in situ tissue profiling. Nat. Methods 16, 987–990 (2019). Article CAS PubMed PubMed Central Google Scholar 10x Genomics. Mouse Brain Serial Sections (Sagittal–Posterior), Spatial Gene Expression Dataset by Space Ranger 1.1.0, 10x Genomics ...
空间转录组技术(spatial transcriptomics)则需要利用常规的原位技术和组学技术两方面的优势。 图3. 单细胞测序技术解决了通量和分辨率的问题 现有的空间转录组技术主要分为两类:一类是基于杂交和成像的方法,例如smFISH,Branched FISH;另一类是基于测序的方法,包括TIVA,ISS,FISSEQ等。smFISH,Branched FISH等靶向方法在...
对于两年以上的甲醛固定石蜡包埋(FFPE)样本,选择哪种空间转录组测序(Spatial transcriptomics)技术取决于几个关键因素,包括样本的保存状态、研究目的和可用技术的特点。[1] 下面简要分析几种主流技术的适用性: 1. NanoString GeoMx DSP: - 优点:NanoString的GeoMx Digital Spatial Profiler非常适合于FFPE样本,因为它不依...