An increasing number of commercially available platforms now facilitate spatially resolved, high-dimensional assessment of gene transcription, known as 'spatial transcriptomics'. Here, we introduce different classes of method, which either record the locations of hybridized mRNA molecules in tissue, image ...
An introduction to spatial transcriptomics for biomedical research. Genome Med. 14, 68 (2022). Article CAS PubMed PubMed Central Google Scholar Mirzazadeh, R. et al. Spatially resolved transcriptomic profiling of degraded and challenging fresh frozen samples. Nat. Commun. 14, 509 (2023). ...
Recent advances in spatial omics have expanded the spectrum of profiled molecular categories beyond transcriptomics. However, many of these technologies are constrained by limited spatial resolution, hindering our ability to deeply characterize intricate
Recent advances in spatially resolved transcriptomics have enabled comprehensive measurements of gene expression patterns while retaining the spatial context of the tissue microenvironment. Deciphering the spatial context of spots in a tissue needs to us
Introduction Transcriptomicsis used to identify the transcriptional activity that is present in cells and tissues (Hegde et al., 2003). Thetranscriptomeconsists of the coding andncRNAsthat are transcribed in cells, tissues, or organs during normal physiological or pathological conditions (Assis et al....
3.3Transcriptomics Thetranscriptomeis the complete set of transcribed genes present within a cell at a given point of time. The first use and definition of the word “transcriptome” date back to 1997 in a work by Velculescu et al.[222], where the authors analysed and characterized the genes...
(BN) construction. We provide an integrative bioinformatics pipeline to explore crucial transcriptional insights from zebrafish starvation-induced transcriptome data in a user-friendly way with minimal programming skill requirements. In this way, many genes, e.g.,skp1,atp5h,ndufb10,rpl5b,zgc:193613...
1. Introduction Bovine coronavirus (BCoV) is one of the major respiratory and enteric viral pathogens of cattle, causing a substantial impact on animal welfare in the beef and dairy industries [1,2,3]. BCoVs, belonging to the familyCoronaviridae, genusBetacoronavirus, are enveloped, positive-sen...
Hepatocellular carcinoma (HCC) often presents with satellite nodules, rendering current curative treatments ineffective in many patients. The heterogeneity of HCC is a major challenge in personalized medicine. The emergence of spatial transcriptomics (ST
In contrast, the other subpopulations were not involved in the maintenance of epicardial integrity but in the formation of the outflow tract (Epi2) and the recruitment of myeloid cells to the developing heart (Epi3). Mantri et al. combined single-cell and spatial transcriptomics (high-...