Epi ATAC What is Epi ATAC? Chromium Epi ATAC (Assay for Transposase Accessible Chromatin) analyzes thousands of unique open chromatin fragments genome-wide with single cell resolution. Find user guidesGet started Documentation We provide resources from protocols, user guides, and free-to-use analysis...
FormerlySingle Cell ATAC What is Epi ATAC? Chromium Epi ATAC (Assay for Transposase Accessible Chromatin) analyzes thousands of unique open chromatin fragments genome-wide with single cell resolution. Find user guidesGet started Documentation We provide resources from protocols, user guides, and free-...
10x Genomics单细胞测序在单个细胞水平进行高通量测序,其能够以单细胞分辨率进行基因表达谱测序、染色质易开放区域测序(ATAC-seq)、V(D)J免疫组库测序和基因组测序等,越来越受研究者的青睐。 单细胞测序适用于绝大部分的细胞类型,包括生殖细胞、胚胎细胞、血细胞、神经细胞、肿瘤细胞、免疫细胞、干细胞等。同时单细胞...
Single-Cell ATAC-Seq: For assessing chromatin accessibility and providing insights into the epigenetic landscape. 3. Is Single-Cell Sequencing Currently Feasible for Plants? Unlike single-cell sequencing in animals, plant single-cell research is still in its infancy. One significant challenge stems fro...
目前的研究多基于 模式植物 ①②③ 单细胞ATAC测序 ①利用转座酶混合液孵育细胞核悬液,转座酶进入细胞核,优先在切割开放染色质区域使DNA片段化,并在DNA片段的末端加上 测序引物;②细胞核裂解释放DNA片段,GEMs中Gel beads溶解,以Read 1N为引物对加好接头的DNA片段进行扩增;③ GEMs 破裂,释放出扩增好的产物 ④...
PBMCs were isolated for single-cell transcriptome and ATAC profiling using the 10x Multiome Genomics Chromium platform. Each cDNA library underwent sequencing for 150 million base pairs. Data normalization followed the Seurat protocol, and cell type annotation relied on cell markers in Seurat. ...
10x官方ATAC教程数据集 打开界面后你可以通过点击“Recent Files”页面中的“ATACTutorial.cloupe”来获取ATAC教程数据集。ATAC教程数据集是根据标准Chromium™ Single-Cell ATAC protocol利用cellranger-atac流程分析人外周血单核细胞得到的结果,流程分析得到的barcode计数为5335。
高通量测序技术的迅速发展为生物研究带来了前所未有的机遇,但传统的Bulk测序手段是针对细胞集合进行测序,而单个细胞特异性的信息往往被掩盖,导致错失很多重要信息。10x Genomics单细胞测序在单个细胞水平进行高通量测序,其能够以单细胞分辨率进行基因表达谱测序、染色质易开放区域测序(ATAC-seq)、V(D)J免疫组库测序和基...
考虑到单细胞多组学技术的快速发展,计划扩展 scPNMF 以适应其他测量其他基因组学特征的技术,例如通过单细胞 ATAC-seq 测量的染色质可及性景观,甚至整合跨多组学数据集的数据 . 另一个注意事项是,scPNMF 中基础选择的多模态测试只考虑了离散的细胞类型,而不是连续的细胞轨迹。 因此,需要其他测试或策略来选择信息基...
What is Epi Multiome ATAC + Gene Expression? Chromium Epi Multiome ATAC + Gene Expression performs direct measurements of 3' gene expression and chromatin accessibility from the same cell. Find user guidesGet started Documentation We provide resources from protocols, user guides, and free-to-use ...