(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开.有关ZFNs...
Custom-designed zinc finger nucleases (ZFNs)--proteins designed to cut at specific DNA sequences--combine the non-specific cleavage domain (N) of Fok I restriction endonuclease with zinc finger proteins (ZFPs). Because the recognition specificities of the ZFPs can be easily manipulated ...
目前的基因编辑技术有ZFNs(zinc-finger nucleases)技术、TALENs(transcription activator-likeeffectornucleases)技术、CRISPR/Cas9技术和BE(base editing)及PE(prime editing)技术。 ZFNs、TALENs和CRISPR/Cas9技术是将DNA打断后依赖非同源末端连接或同源定向重组修复的方式进行修复。 BE可以将某一特定位点的特定碱基对替换为...
Zinc Finger Nucleases (ZFNs) are a programmable DNA binding protein system composed of zinc finger proteins fused to a bacterial nuclease. They enable site-specific double stranded DNA breaks for gene editing by recognizing specific DNA sequences within the genome. ...
Zinc finger nucleases are designed to be like heat-seeking missiles, precisely targeted to find and cut specific sequences of DNA. 锌指核酸酶(zincfingernucleases,ZFNs)被设计为类似于热跟踪导弹,精确地导向以便找到并切割特异性DNA序列。 bbs.bioon.net 4. The idea is that these zinc finger proteins ...
Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell′s natural...
A significant advancement toward this objective was achieved with the advent of a novel technology that enables high-frequency and high-fidelity genome editing via the application of custom designed zinc finger nucleases (ZFNs). A ZFN is a chimeric protein that consists of the non-specific ...
1.The newly developed technology,zinc finger nucleases(ZFNs) targeted genome engineering,shows promise in improving the efficiency of gene targeting by introducing DNA double-strand breaks(DSB) in target genes,which then stimulate the cell\'s endogenous homologous recombination(HR) machinery.锌指核酸酶...
Genome Engineering With Zinc-Finger Nucleases Zinc-finger nucleases (ZFNs) are targetable DNA cleavage reagents that have been adopted as gene-targeting tools. ZFN-induced double-strand breaks are subj... Carroll,D. - 《Genetics》 被引量: 1504发表: 2011年 Targeted chromosomal cleavage and mutagene...
求翻译:Zinc-Finger Nucleases (ZFNs)是什么意思?待解决 悬赏分:1 - 离问题结束还有 Zinc-Finger Nucleases (ZFNs)问题补充:匿名 2013-05-23 12:21:38 正在翻译,请等待... 匿名 2013-05-23 12:23:18 核酸J Zinc-Finger ( ZFNs ) 匿名 2013-05-23 12:24:58 锌手指Nucleases (ZFNs) 匿名 ...