(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开.有关ZFNs...
(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开.有关ZFNs...
Custom-designed zinc finger nucleases (ZFNs)--proteins designed to cut at specific DNA sequences--combine the non-specific cleavage domain (N) of Fok I restriction endonuclease with zinc finger proteins (ZFPs). Because the recognition specificities of the ZFPs can be easily manipulated ...
作者: 目前的基因编辑技术有ZFNs(zinc-finger nucleases)技术、TALENs(transcription activator-likeeffectornucleases)技术、CRISPR/Cas9技术和BE(base editing)及PE(prime editing)技术。 ZFNs、TALENs和CRISPR/Cas9技术是将DNA打断后依赖非同源末端连接或同源定向重组修复的方式进行修复。 BE可以将某一特定位点的特定碱基对...
Zinc Finger Nucleases (ZFNs) are a programmable DNA binding protein system composed of zinc finger proteins fused to a bacterial nuclease. They enable site-specific double stranded DNA breaks for gene editing by recognizing specific DNA sequences within the genome. ...
Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell′s natural...
Zinc finger nucleases are designed to be like heat-seeking missiles, precisely targeted to find and cut specific sequences of DNA. 锌指核酸酶(zincfingernucleases,ZFNs)被设计为类似于热跟踪导弹,精确地导向以便找到并切割特异性DNA序列。 bbs.bioon.net 4. The idea is that these zinc finger proteins ...
1.The newly developed technology,zinc finger nucleases(ZFNs) targeted genome engineering,shows promise in improving the efficiency of gene targeting by introducing DNA double-strand breaks(DSB) in target genes,which then stimulate the cell\'s endogenous homologous recombination(HR) machinery.锌指核酸酶...
A significant advancement toward this objective was achieved with the advent of a novel technology that enables high-frequency and high-fidelity genome editing via the application of custom designed zinc finger nucleases (ZFNs). A ZFN is a chimeric protein that consists of the non-specific ...
求翻译:Zinc-Finger Nucleases (ZFNs)是什么意思?待解决 悬赏分:1 - 离问题结束还有 Zinc-Finger Nucleases (ZFNs)问题补充:匿名 2013-05-23 12:21:38 正在翻译,请等待... 匿名 2013-05-23 12:23:18 核酸J Zinc-Finger ( ZFNs ) 匿名 2013-05-23 12:24:58 锌手指Nucleases (ZFNs) 匿名 ...