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锌指核酸酶(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开...
网络释义 1. 锌指核酶技术 锌指核酶技术(Zinc-finger nucleases)、定向蛋白质组学技术(Targeted proteomics)、单分子结构测定技术(Single-molecu… www.lifeomics.com|基于8个网页 2. 锌指核酸酶 锌指核酸酶(zinc-finger nucleases)与转录激活因子样效应物核酸酶(transcription activator–like effector nucleases, TALEN…...
These are limited-specificity nucleases that can recognize certain DNA sequences and cause small deletions at selected targets. The Zn fingers prefer guanine-rich tracts, particularly 5′-GNN-3′ triplets. Each nuclease unit may have three fingers and each grabs one triplet, i.e., a total of...
目前的基因编辑技术有ZFNs(zinc-finger nucleases)技术、TALENs(transcription activator-like effector nucleases)技术、CRISPR/Cas9技术和BE(base editing)及PE(prime editing)技术。ZFNs、TALENs和CRISPR/Cas9技术是将DNA打断后依赖非同源末端连接或同源定向重组
英文: Construction of Artificial Zinc Finger Libraries and Application in Screening Zinc Finger Nucleases中文: 人工锌指蛋白随机库的构建及其在锌指核酸酶筛选中的应用 英文: The catalytic properties of zinc oxide catalysts from zinc oxide, zinc acetate calcined at 600℃ and zinc carbonate prepared through...
1.2.2.1 Zinc-Finger Nucleases ZFNs are, by nature, engineered nucleases that link the DNA-binding domain of eukaryotic transcription factors/zinc-finger proteins with the nuclease domain of an endonuclease, generally FokI restriction enzyme (Fig. 1.2A). In this approach, a tandem array of Cys2-...
Zinc-finger nucleasesNatalie de Souza Nature Methods volume 8, page 43 (2011)Cite this article 2396 Accesses 4 Citations 6 Altmetric Metrics details Genome-engineering tools with improved design and efficiency will become widely used. This is a preview of subscription content, access via your ...
A significant advancement toward this objective was achieved with the advent of a novel technology that enables high-frequency and high-fidelity genome editing via the application of custom designed zinc finger nucleases (ZFNs). A ZFN is a chimeric protein that consists of the non-specific ...
Zinc finger nucleases are designed to be like heat-seeking missiles, precisely targeted to find and cut specific sequences of DNA. 锌指核酸酶(zincfingernucleases,ZFNs)被设计为类似于热跟踪导弹,精确地导向以便找到并切割特异性DNA序列。 bbs.bioon.net 4. The idea is that these zinc finger proteins ...