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A significant advancement toward this objective was achieved with the advent of a novel technology that enables high-frequency and high-fidelity genome editing via the application of custom designed zinc finger nucleases (ZFNs). A ZFN is a chimeric protein that consists of the non-specific ...
目前的基因编辑技术有ZFNs(zinc-finger nucleases)技术、TALENs(transcription activator-likeeffectornucleases)技术、CRISPR/Cas9技术和BE(base editing)及PE(prime editing)技术。 ZFNs、TALENs和CRISPR/Cas9技术是将DNA打断后依赖非同源末端连接或同源定向重组修复的方式进行修复。 BE可以将某一特定位点的特定碱基对替换为...
关于zinc-finger nucleases热点的两篇NATURE文章
1.2.2.1 Zinc-Finger Nucleases ZFNs are, by nature, engineered nucleases that link the DNA-binding domain of eukaryotic transcription factors/zinc-finger proteins with the nuclease domain of an endonuclease, generally FokI restriction enzyme (Fig. 1.2A). In this approach, a tandem array of Cys2-...
锌指核酸酶(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开...
关于zinc-finger nucleases热点的两篇NATURE文章 下载提醒:APP中打开可直接下载,点击下载 High-frequency modification of plant genes using engineered zinc-finger nucleases.pdf Targeted mutagenesis in zebrafish using customized zinc-finger nucleases.pdf 返回小木虫查看更多分享...
Zinc-finger nucleasesNatalie de Souza Nature Methods volume 8, page 43 (2011)Cite this article 2396 Accesses 4 Citations 6 Altmetric Metrics details Genome-engineering tools with improved design and efficiency will become widely used. This is a preview of subscription content, access via your ...
The recent discovery and construction of zinc finger nucleases (ZFN) seem promising in combating HIV. Comprising a DNA binding domain and a cleavage domain, the ZFN works by cleaving the targeted region and introducing a double strand break (DSB) near the intended recombination site, which ...
1. 锌指核酶技术 锌指核酶技术(Zinc-finger nucleases)、定向蛋白质组学技术(Targeted proteomics)、单分子结构测定技术(Single-molecu… www.lifeomics.com|基于8个网页 2. 锌指核酸酶 锌指核酸酶(zinc-finger nucleases)与转录激活因子样效应物核酸酶(transcription activator–like effector nucleases, TALEN… ...