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其中,zinc finger nuclease(ZFN)是一种利用人工设计的锌指蛋白酶(zinc finger proteins,ZFPs)结合核酸递质实现靶向剪切基因组DNA的技术。它是一种高效、精准、灵活的基因编辑工具,被广泛应用于动植物、细菌等生物体基因组编辑中。 一、ZFN的原理 Zinc finger proteins是一类与DNA结合的蛋白质,具有不同结构的指环域,...
An improved zinc-finger nuclease architecture for highly specific genome editing. Nat Biotechnol 2007;25:778-85.Miller,J.C., Holmes,M.C., Wang,J., Guschin,D.Y., Lee,Y.L., Rupniewski,I., Beausejour,C.M., Waite,A.J., Wang,N.S., et al. (2007) An improved zinc-finger nuclease...
研究人员使用 Zinc Finger Nuclease 技 术成功培育首个靶基因敲除大鼠 来自 Milwaukee 的消息 , 威斯康新医学院 , Sangamo Biosciences , Sigma-Aldrich , Open Monoclonal Technology 以及 INSERM 公司联合宣布使用 zinc finger nuclease (ZFN)技术成功培育出首 只靶基因敲除大鼠. 在 7 月 24 日出版的《Science》...
摘要:来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用zinc finger nuclease (ZFN)技术成功培育出首只靶基因敲除大鼠。 来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用...
摘要:来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用zinc finger nuclease (ZFN)技术成功培育出首只靶基因敲除大鼠。 来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用...
In initial studies, we systematically varied the length and composition of the linker connecting the zinc finger and nuclease domains, and determined the impact of these changes on the cleavage efficiency and optimal target arrangement of the resultant ZFN (which functions as a dimer). It was ...
Enhancing zinc-finger-nuclease activity with improved obligate heterodimeric architectures Zinc-finger nucleases (ZFNs) drive efficient genome editing by introducing a double-strand break into the targeted gene. Cleavage is induced when two custo... Y Doyon,TD Vo,MC Mendel,... - 《Nature Methods》...
R. et al. Chemically regulated zinc finger transcription factors. J. Biol. Chem. 275, 32617–32627 (2000). Article CAS PubMed Google Scholar Gross, D. S. & Garrard, W. T. Nuclease hypersensitive sites in chromatin. Annu. Rev. Biochem. 57, 159–197 (1988). Article CAS PubMed ...
1(A)). The zinc finger proteins provide site specific targeting as they each recognize a 3–4 base pair DNA sequence.6,12 In ZFNs, a chain of zinc finger proteins are utilized to recognize a longer, more specific locus within the genome. The nuclease commonly used in ZFN technology, ...