Zinc Finger Nuclease (ZFN) - Modify human genome precisely and efficientlyPriyanka Chaudhary
Zinc-finger nucleases DSB: DNA double strand breaks NHEJ: Non-homologous end joining TALEN: Transcription activator-like effector nuclease SCID: Severe combined immunodeficiency NKC: Natural killer cells ZDD: Zinc-binding dimerization domain NBR: Nonamer binding region PALS: Periarteriolar ly...
其中,zinc finger nuclease(ZFN)是一种利用人工设计的锌指蛋白酶(zinc finger proteins,ZFPs)结合核酸递质实现靶向剪切基因组DNA的技术。它是一种高效、精准、灵活的基因编辑工具,被广泛应用于动植物、细菌等生物体基因组编辑中。 一、ZFN的原理 Zinc finger proteins是一类与DNA结合的蛋白质,具有不同结构的指环域,...
This Kalorama report, Gene Editing Markets (CRISPR-based, Zinc Finger Nuclease [ZFN], TALEN, Other), provides global Revenues and Forecast for the gene editing market by type of product including consumables, instruments, services, cell-lines, animal model...
Granja S, Marchiq I, Baltazar F, Pouyssegur J. Gene disruption using zinc finger nuclease technology. Methods Mol Biol, 2014, 1165: 253-260Granja S, Marchiq I, Baltazar F, Pouyssegur J. Gene disruption using zinc finger nuclease technology. Methods Mol Biol 2014; 1165: 253-260....
摘要:来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用zinc finger nuclease (ZFN)技术成功培育出首只靶基因敲除大鼠。 来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用...
研究人员使用 Zinc Finger Nuclease 技 术成功培育首个靶基因敲除大鼠 来自 Milwaukee 的消息 , 威斯康新医学院 , Sangamo Biosciences , Sigma-Aldrich , Open Monoclonal Technology 以及 INSERM 公司联合宣布使用 zinc finger nuclease (ZFN)技术成功培育出首 只靶基因敲除大鼠. 在 7 月 24 日出版的《Science》...
摘要:来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用zinc finger nuclease (ZFN)技术成功培育出首只靶基因敲除大鼠。 来自Milwaukee的消息,威斯康新医学院、Sangamo Biosciences、Sigma-Aldrich、Open Monoclonal Technology以及INSERM公司联合宣布使用...
Zinc-finger nucleases allow targeted genetic modification at loci chosen by the investigator, but the extent of their off-target activity—which could be toxic to cells—has not been evaluated experimentally on a genome-wide scale. Gabriel et al. documen
Attenuation of Zinc Finger Nuclease Toxicity by Small- Molecule Regulation of Protein Levels Shondra M. Pruett-Miller 1,2 , David W. Reading 1,2 , Shaina N. Porter 1,2 , Matthew H. Porteus 1,2 * 1Department of Pediatrics, University of Texas Southwestern Medical Center, Dallas, Texas, ...