锌指核酸酶(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割开...
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锌指核酸酶(zinc-finger nucleases)与转录激活因子样效应物核酸酶(transcription activator–like effector nucleases, TALEN… www.lifeomics.com|基于3个网页 3. 锌指核酸酶技术 利用锌指核酸酶技术(zinc-finger nucleases)在一细胞期的胚胎中进行基因打靶是一种高效的诱变方式,这种方法可以应用在 … ...
Zinc finger nucleases are associations of the Zinc finger with, e.g., a FokI restriction endonuclease (target GGATG(N) 9/13 ) and can thus be targeted to specific mammalian genes (Porteus MH 2006 Mol Ther 13:438) or can be used to repair specific defective genes in plants by targeted ...
9.锌指核酸酶(zincfinger nucleases,ZFNs)是科学家为特异性切割DNA构建的工具,由一个DNA识别域和一个DNA剪切域构成.DNA识别域含一到三个锌指蛋白,每个锌指蛋白可以结合一个DNA上的碱基三联体,如GAC,ATA等,几个锌指蛋白串联,可以实现对特异DNA序列的识别;两个DNA剪切域临近时可以产生核酸内切酶活性,将接触的DNA切割...
目前的基因编辑技术有ZFNs(zinc-finger nucleases)技术、TALENs(transcription activator-like effector nucleases)技术、CRISPR/Cas9技术和BE(base editing)及PE(prime editing)技术。ZFNs、TALENs和CRISPR/Cas9技术是将DNA打断后依赖非同源末端连接或同源定向重组
Zinc Finger Nucleases (ZFNs) are a programmable DNA binding protein system composed of zinc finger proteins fused to a bacterial nuclease. They enable site-specific double stranded DNA breaks for gene editing by recognizing specific DNA sequences within the genome. ...
英文: Construction of Artificial Zinc Finger Libraries and Application in Screening Zinc Finger Nucleases中文: 人工锌指蛋白随机库的构建及其在锌指核酸酶筛选中的应用 英文: The catalytic properties of zinc oxide catalysts from zinc oxide, zinc acetate calcined at 600℃ and zinc carbonate prepared through...
1.The newly developed technology,zinc finger nucleases(ZFNs) targeted genome engineering,shows promise in improving the efficiency of gene targeting by introducing DNA double-strand breaks(DSB) in target genes,which then stimulate the cell\'s endogenous homologous recombination(HR) machinery.锌指核酸酶...
Zinc-finger nucleasesNatalie de Souza Nature Methods volume 8, page 43 (2011)Cite this article 2396 Accesses 4 Citations 6 Altmetric Metrics details Genome-engineering tools with improved design and efficiency will become widely used. This is a preview of subscription content, access via your ...