The proteomic profile of the microglial-derived exosomes was characterised using liquid chromatography-tandem mass spectrometry (LC/MS/MS) and the vesicles were found to be associated with proteins involved in cellular architecture, metabolism, protein synthesis and protein degradation including ??-actin,...
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Therefore, LRP6 seemed to be internalized and recycled in a Wnt-3a-dependent manner that was not dependent on protein synthesis. Download: Download high-res image (806KB) Download: Download full-size image Figure 1. Wnt-3a Induces LRP6 Internalization (A) HEK293 cells expressing LRP6-GFP were...
Recombinant human WNT3A protein promotes myogenesis of hESC-derived PDGFRA1 cells. To further verify the role of WNT3A protein on myogenic commitment of PDGFRA1 cells, we have cultured the cells in induction medium containing varying amounts (0, 10, 50, and 100 ng/mL) of human recombinant ...
The injury of vascular endothelial cells is a crucial factor in the development of diabetic retinopathy (DR). PDLIM1 (a member of the PDZ and LIM protein family) has been reported to exert an essential function in vascular diseases. This study aimed to e
synthesis was analyzedby melanincontentand tyrosinaseactivity.The effectof Wnt3aonmRNAand protein levelsofMITEDCT TRP1,andtyrosinase was analyzedby RT-PCRandWesternBlot. (3)Used“iMC23”as animmature melancoyte cellmodel,toanalyze theeffectof
However, in the presence of Wnt ligands, the binding of Wnt ligands to the Frizzled receptor and LRP5/6 co-receptor activates Dishevelled protein, which inhibits the protein destruction complex. Subsequently, the stabilized β-catenin protein leads to its nuclear translocation and transactivates Wnt...
TYR)活性;Masson—Fontanas银染色法观察黑素形成情况;RT-PCR检测酪氨酸酶相关蛋白1(tyrosinase-relatedprotein1, TRP.1)、TYR基因的表达;Westernblot检测TRP一1、TYR蛋白的表达水平。结果 L—DOPA测定酪氨酸酶活性结果显示 Wnt3a+sFRP5处理组TYR活性低于Wnt3a处理组,差异有统计学意义(P<0.05);Masson—Fontanas银染...
Nonunion and delayed union are common complications of diabetes mellitus that pose a serious health threat to people. There are many approaches that have been used to improve bone fracture healing. Recently, exosomes have been regarded as promising medic
论文题目:A Fusion of Artificial Spidroin and Mussel Foot Protein That Retains High Adhesion and Natural Glue Formation Ability 学校:上海星河湾双语学校 参赛学生:杨珺萌 指导老师:Yongsheng Bai 论文题目:Identification and Genetic Signatur...