Whole-Genome Shotgun Metagenomic Sequencing Reveals Shifts in the Skin Microbiome and Bacteriophages of Psoriasis: An Extended Analysis of Published Datadoi:10.1177/24755303241242357Nong, YvonneWalsh, Dana M.Maloh, JessicaDadlani, ManojSivamani, Raja...
Whole genome shotgun metagenomic sequences from the proximal colon microbiota in response to helminth parasites 来自 ars.usda.gov 喜欢 0 阅读量: 45 作者: R Li 摘要: Helminth parasites ensure their survival by regulating host immunity through mechanisms that dampen inflammation. These properties have...
These results underscore the importance of measuring microbial load, either using deep shotgun sequencing, or, as we propose, combining low coverage shotgun data with amplicon data in a cost-effective hybrid approach. Methods Sampling, processing of plants, and metagenomic library preparation Plants ...
In a shotgun metagenomic sequencing experiment, libraries were constructed using the xGen DNA Library EZ Kit and 1 ng of DNA derived from a mock metagenome DNA standard (ATCC, MSA-1000™) with varying GC content (30–68.8%). The kit provided comprehensive normalized coverage across di...
aRNA (rRNA) gene sequence (16S) and taxonomic profiles, whole-genome shotgun (WGS) or metagenomic sequencing of whole community DNA, and alignment of the assembled sequences to the reference RNA (rRNA)基因序列(16S)和分类学外形,完全染色体猎枪(WGS)或metagenomic被装配的序列的程序化整体社区脱氧核糖...
Use of Metagenomic Shotgun Sequencing Technology to Detect Foodborne Pathogens within the Microbiome of the Beef Production Chain. Appl. Environ. Microbiol. 2016, 82, 2433–2443. [CrossRef] [PubMed] 106. Huang, A.D.; Luo, C.; Pena-Gonzalez, A.; Weigand, M.R....
L. Centrifuge: rapid and sensitive classification of metagenomic sequences. Genome Res. 26, 1721–1729 (2016). CAS PubMed PubMed Central Google Scholar Médigue, C., Cole, S. T., Camus, J.-C. & Pryor, M. J. Re-annotation of the genome sequence of Mycobacterium tuberculosis H37Rv. ...
23:541. 05103-0 https://doi.org/10.1186/s12859-022- 57 How many shotgun metagenomic WGS samples can be multiplexed on a single SMRT Cell? The overall goals of your project will determine the needed coverage depth General guidance for multiplexing shotgun metagenomic samples1 • ...
(EV-D68) infections. During the outbreak, we collected nasopharyngeal swab specimens from patients in the Lower Hudson Valley of New York. Here, we conduct a retrospective study on the genomic diversity of EV-D68 strains. We first employ a metagenomic shotgun sequencing protocol on a total of...
We sequenced the genome of a male GSM using a whole-genome shotgun strategy. Cleaned-up data provided 146-fold average coverage across 3.05 Gb of assembled sequence (Supplementary Fig. 2 and Supplementary Tables 1 and 2), with N50 values of 25.5 kb and 1.55 Mb, respectively, for contigs ...