Introduction: Whole Genome Shotgun Sequencing (WGS) of the microbiota is a powerful technique that provides an unbiased look at the relative abundance of the gut microbiome, but the analysis of the large data sets requires a bioinformatician using software executed on high-powered servers. This ...
In this study, we characterized the microbiota in the porcine proximal colon in response to Trichuris suis (whipworm) infection using 16S rRNA gene-based and whole-genome shotgun (WGS) sequencing. A 21-day T. suis infection in four pigs induced a significant change in the composition of the...
Metagenomics Tools : BACS / Fosmid Libraries Whole Genome Shotgun SequencingApprill, Amy
Microorganisms from all domains of life establish associations with plants. Although some harm the plant, others antagonize pathogens or prime the plant immune system, support the acquisition of nutrients, tune plant hormone levels, or perform additional
The application of whole-genome shotgun sequencing to microbial communities represents a major development in metagenomics, the study of uncultured microbes via the tools of modern genomic analysis. In the past year, whole-genome shotgun sequencing projects of prokaryotic communities from an acid mine...
Keywords: antimicrobial resistance; surveillance; foodborne pathogens; whole genome sequencing; metagenomics 1. Introduction Antibiotics have been used in human and veterinary medicine for more than 70 years and have greatly contributed to tackling pathogenic bacteria and protecting ...
africanum in sputum samples using shotgun metagenomics on a benchtop sequencer. PeerJ 2, e585 (2014). PubMed PubMed Central Google Scholar Phelan, J. E. et al. Recombination in pe/ppe genes contributes to genetic variation in Mycobacterium tuberculosis lineages. BMC Genomics 17, 151 (2016...
Taxonomic profiling of microbial communities is often performed using small subunit ribosomal RNA (SSU) amplicon sequencing (16S or 18S), while environmental shotgun sequencing is often focused on functional analysis. Large shotgun datasets contain a significant number of SSU sequences and these can ...
如题：求大神指导一下whole metagenomics shotgun sequencing,技术实验流程,分析流程，多谢！
1 Refer to https://github.com/PacificBiosciences/pb-metagenomics-tools for details about specific PB-metagenomics-tools pipelines. 56 HiFi WGS data analysis recommendations for small genomes – Shotgun metagenomics applications (cont.) Use HiFi-MAG-Pipeline to obtain high-quality metagenome...