Here, we sequenced the faecal microbiome of a local pig breed by using whole-metagenome sequencing. Metagenomic data revealed that the faecal microbiome consists of a complex and intricate admixture of microbes belonging to all domains of life, including viruses. Most of sequencing reads were mapped...
In this study, we performed whole metagenome shotgun sequencing of fecal samples to enhance the detection of rare bacterial species and increase genome sequence coverage. Additionally, we employed multiple bioinformatics approaches to investigate the potential targets of the microbiome as an indicator of...
Use of Metagenomic Shotgun Sequencing Technology to Detect Foodborne Pathogens within the Microbiome of the Beef Production Chain. Appl. Environ. Microbiol. 2016, 82, 2433–2443. [CrossRef] [PubMed] 106. Huang, A.D.; Luo, C.; Pena-Gonzalez, A.; Weigand, M.R.; T...
3 Size selection is not required for microbial WGS and metagenomic shotgun libraries where retention of shorter fragments is desired. 13 SPK 3.0 workflow overview for WGS & metagenomic sequencing applications SPK 3.0 library prep workflow can optionally be automated to enable higher sample...
Strain-level microbial epidemiology and population genomics from shotgun metagenomics. Nat Methods. 2016;13(5):435–8. Article CAS PubMed Google Scholar Download references Acknowledgements Part of the research described in this publication was carried out at the Jet Propulsion Laboratory, California ...
Whole metagenome shotgun sequencing is a powerful approach for assaying many aspects of microbial communities, including the functional and symbiotic potential of each contributing community member. The research community currently lacks tools that efficiently align DNA reads against protein references, the ...
While microbial eukaryotes are critical to these communities, they are challenging to study with shotgun sequencing techniques and are therefore often excluded. Results Here, we present EukDetect, a bioinformatics method to identify eukaryotes in shotgun metagenomic sequencing data. Our approach uses a...
In this study, we performed whole metagenome shotgun sequencing of fecal samples to enhance the detection of rare bacterial species and increase genome sequence coverage. Additionally, we employed multiple bioinformatics approaches to investigate the potential targets of the microbiome as an indicator of...
Wholemetagenome shotgun sequencing of bone marrow samples in patients with treatment-nave ALL (n=6) was performed for untargeted investigation of bacterial and viral DNA. The control group consisted of healthy children (n=4) and children with non-oncologic diseases (n=2) unde...
Motivation: Over the recent years, the field of whole-metagenome shotgun sequencing has witnessed significant growth owing to the high-throughput sequencing technologies that allow sequencing genomic samples cheaper, faster and with better coverage than before. This technical advancement has initiated the...