Whole-Genome Shotgun Metagenomic Sequencing Reveals Shifts in the Skin Microbiome and Bacteriophages of Psoriasis: An Extended Analysis of Published Datadoi:10.1177/24755303241242357Nong, YvonneWalsh, Dana M.Maloh, JessicaDadlani, ManojSivamani, Raja...
aRNA (rRNA) gene sequence (16S) and taxonomic profiles, whole-genome shotgun (WGS) or metagenomic sequencing of whole community DNA, and alignment of the assembled sequences to the reference RNA (rRNA)基因序列(16S)和分类学外形,完全染色体猎枪(WGS)或metagenomic被装配的序列的程序化整体社区脱氧核糖...
In this study, we characterized the microbiota in the porcine proximal colon in response to Trichuris suis (whipworm) infection using 16S rRNA gene-based and whole-genome shotgun (WGS) sequencing. A 21-day T. suis infection in four pigs induced a significant change in the composition of the...
In a shotgun metagenomic sequencing experiment, libraries were constructed using the xGen DNA Library EZ Kit and 1 ng of DNA derived from a mock metagenome DNA standard (ATCC, MSA-1000™) with varying GC content (30–68.8%). The kit provided comprehensive normalized coverage across di...
3. Whole Metagenome Sequencing Metagenomic approaches are culture-independent alternatives for direct characterization of the microbiota of food, water, fecal, soil, or environmental samples, among others [98]. The term metagenome sequencing is used for two different high-throug...
Whole genome sequencing (WGS) of Mycobacterium tuberculosis has rapidly progressed from a research tool to a clinical application for the diagnosis and management of tuberculosis and in public health surveillance. This development has been facilitated by drastic drops in cost, advances in technology and...
thaliana plants, we took a metagenomic shotgun sequencing approach to analyze entire wild phyllospheres. Because plant genomic DNA was expected to dominate such samples, we initially attempted to develop a protocol to enrich the microbial component. To this end, we used genomic DNA from sterilely...
Next-generation sequencing for beginners Curious about using NGS in your research? Learn the basics of next-generation sequencing and find tips for getting started. Learn about NGS Techniques Sequencing DNA Sequencing RNA Sequencing High-Throughput Sequencing Library Preparation Microarrays Multiomics...
OPEN articles Whole-genome sequencing of the snub-nosed monkey provides insights into folivory and evolutionary history Xuming Zhou1,2,16, Boshi Wang1,2,16, Qi Pan3,16, Jinbo Zhang3, Sudhir Kumar4, Xiaoqing Sun3, Zhijin Liu1, Huijuan Pan5, Yu Lin3, Guangjian Liu1,2, Wei Zhan3, ...
3 Size selection is not required for microbial WGS and metagenomic shotgun libraries where retention of shorter fragments is desired. 13 SPK 3.0 workflow overview for WGS & metagenomic sequencing applications SPK 3.0 library prep workflow can optionally be automated to enable higher sample...