Amylin dosing resulted in neuronal activation as measured by an increase in c‐Fos labelled cells in 20 brain regions, altogether making up the circuitry of neuronal appetite regulation (e.g., area postrema (AP), nucleus of the solitary tract (NTS), parabrachial nucleus (PB), and central ...
Some of, but not all, the brain regions in the EE-treated mice showing enhanced MEMRI signal intensity had accompanying increases in c-Fos expression. The EE-treated mice were also found to have significantly increased overall amount of food consumption, decreased body weight gain and upregulated...
An additional method that allows for the evaluation of behavior-induced neural activity in rodents is based on the use of genes regulated by neuronal activity, such as Fos. Fos is a transcription factor induced by neural activity resulting from emotional arousal or sensory signals [190,191]. Imm...
DREAMM is thus the first technique that provides simultaneously quantitative, dynamic, whole-brain and regionally unbiased, cell type-specific functional circuit mapping captured during the awake, freely moving state. DREAMM affords the unique flexibility of combining multiple strategies—molecular, viral,...
Mapping immediate early gene (IEG) expression across intact brains is becoming a popular approach for identifying the brain-wide activity patterns underlying behavior. Registering whole brains to an anatomical atlas presents a technical challenge that has predominantly been tackled using automated voxel-ba...
mapping of the spatial organization of molecularly defined cell types in complex tissues1,23, including several brain regions (for example, refs.1,2,3,4,5,6,7,8,9,10,11). However, a comprehensive cell atlas of the whole brain is still missing. Here we imaged a panel of more than 1...
compared to the PC group. C-Fos results highlighted the role of the prefrontal cortex,dorsal striatum, anterodorsalthalamusand CA3 in the PC group, compared to the OPC, RM5 and RM8 groups. The animals were able to find the escape platform even when only a portion of the space where the...
Whole-brain activity mappingBackground:Cortical spreading depression (CSD) is an electrophysiological event underlying migraine aura. Traditional CSD models are invasive and often cause injuries. The aim of the study was to establish a minimally invasive optogenetic CSD model and identify the active ...
We developed an automated whole-brain mapping pipeline to identify brain circuits underlying pair-bonding behavior. We identified bonding-related c-Fos induction in 68 brain regions clustered in seven major brain-wide neuronal circuits. These circuits include known regulators of bondin...
To investigate the brain mechanisms of the impaired social behavior in the Cntnap2 KO mice, we used serial two-photon (STP) tomography-based mapping of whole brain activity. STP tomography images the entire mouse brain at cellular resolution with near complete 3D ...