Exome sequencing is a targeted sequencing approach that is restricted to the protein-coding regions of genomes. The exome is estimated to encompass approximately 1% of the genome, yet contains approximately 85% of disease-causing mutations [1]. For genetic re...
Learn about whole exome sequencing (WES), a targeted next generation sequencing method that identifies all the protein-coding genes (exons) in the genome.
Next-generation and whole-genome se- quencing in the diagnostic clinical mi- crobiology laboratory. Eur J Clin Micro- biol Infect Dis 2012;31:1719-26.Dunne WM Jr, Westblade LF, Ford B. 2012. Next-generation and whole- genome sequencing in the diagnostic clinical microbiology laboratory. Eur...
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Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
sites of T-DNA in transgenic plants, the safety of transgenic plants at the genomic level can be assessed.ResultsIn this study, we performed genome resequencing of Pb29, a transgenic high-resistance poplar 741 line that has been commercialized, using next-generation and Nanopore sequencing. The...
sequencing methods. Next-generation sequencing approaches are characterized generally by the massively parallel production of short sequences, in which multiple DNA fragments are generated simultaneously and in sufficient quantity to redundantly represent every base in the target genome. Although such ...
Using whole-genome next-generation sequencing (NGS), researchers at the Central European Institute of Technology at Masaryk University in the Czech Republic—in collaboration with the EMBL Genomics Core Facility, Heidelberg,...
French CE, Delon I, Dolling H, et al; NIHR BioResource—Rare Disease; Next Generation Children Project. Whole genome sequencing reveals that genetic conditions are frequent in intensively ill children. Intensive Care Med. 2019;45(5):627-636. doi:10.1007/s00134-019-05552-xPub...
Next generation sequencing analyses of AM have either involved targeted or exome sequencing7,8,9, or whole-genome sequencing (WGS) of small numbers (35 or fewer) of tumors10,11,12,13. These studies have shown that AM is distinctive, with a lower number of SNV/indel mutations and higher ...