Whole-genome re-sequencing This review comes from a themed issue on Genomes and evolution EditedBentley, David RTylerSmith, ChrisPreworski, Molly
sectioned by a cryostat, and stained with hematoxylin and eosin. We performed macrodissection to enrich the tumor fraction relative to the dominant stromal component and other normal cells. DNA was extracted using a general protocol for genome sequencing. Preparation of ...
Whole-genome sequencing (WGS) is widely employed to diagnose rare [31,32,33,34,35] and undiagnosed diseases [36] and identify actionable cancer drivers and signatures. The different clinical applications and the type of analyses that are implicated in the diagnostics are shown in Fig.2. There ...
Mycobacterium tuberculosis and whole-genome sequencing: how close are we to unleashing its full potential? Clin. Microbiol. Infect. 24, 604–609 (2018).An extensive review of the literature outlining the potential of WGS for TB research and clinical use. CAS PubMed Google Scholar Lipworth, S...
Demographic history, selection and functional diversity of the canine genome. Nat Rev Genet. 2017;18:705–20. Article CAS PubMed Google Scholar Groeneveld LF, Lenstra JA, Eding H, Toro MA, Scherf B, Pilling D, et al. Genetic diversity in farm animals--a review. Anim Genet. 2010;41(...
Peer Review reports Background Chicken (Gallus gallus), the most widely distributed livestock species globally, is valuable for providing animal protein to the increasing human populace and also can be used as an excellent animal model for scientific research [1]. Since the domestication of the red...
Whole genome sequencing provides the most comprehensive collection of an individual's genetic variation. With the falling costs of sequencing technology, we envision paradigm shift from microarray-based genotyping studies to whole genome sequencing. We review methodologies for whole genome sequencing. There...
Glioblastoma (GBM) has one of the worst 5-year survival rates of all cancers. While genomic studies of the disease have been performed, alterations in the non-coding regulatory regions of GBM have largely remained unexplored. We apply whole-genome sequencing (WGS) to identify non-coding mutation...
However, realizing the potential benefit of such sequencing will require a concerted effort by science, medicine, law, and management. In this review, we discuss current approaches to decoding the 6 billion-letter genetic code of a whole genome in a clinical context, give current examples of ...
Here, we perform genome sequencing of 588B. napusaccessions and transcriptome sequencing of 11 tissues from twoB. napusaccessions having different seed quality. The large number of variations identified not only provide insight into the origin and evolutionary history ofB. napus, identifying genetic lo...