Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
Generate complete genome assemblies using long nanopore sequencing reads. Resolve structural variants, repeats, phasing, and base modifications.
Whole-genome sequencing (WGS) techniques have evolved from an expensive luxury typing method affordable only to a few institutions to a common tool for routine analysis in public health microbiology. In particular, improvements in pathogen source tracking, determination of phy...
the genome of which was sequenced to produce the first reference assembly, including the mitochondrial genome7. To obtain the present new reference assembly, we used these recent data based on the Illumina shotgun-sequencing technology and the present new...
Recently developed techniques using Non-negative Matrix Factorization (NMF)5to perform unsupervised analysis of somatic mutation data has enabled the unbiased discovery of genome-wide mutational patterns in multiple tumour types4,6,7. One such study, by Alexandrovet al., analysed 28 CLL WGS and 103...
Currently there is an urgent need for reliable sequencing techniques to determine whether resistant variants influence treatment efficacy. Whole genome sequencing (WGS) directly from clinical samples can contribute significantly to the understanding of clinical population structures, including minority ...
BackgroundSince the introduction of next-generation sequencing (NGS) techniques, whole-exome sequencing (WES) and whole-genome sequencing (WGS) have not on... F Boeykens,SFM Bhatti,LBBJG Peelman - 《Bmc Bioinformatics》 被引量: 0发表: 2023年 Occurrence, genetic diversity, and antimicrobial res...
Genome stability research Evolutionary biology research Different DNA Methylation Detection Techniques 450K/850k This technology scans and detects approximately 450K and 850K important CpG sites known in the genome through chip scanning. It exhibits high accuracy and repeatability, but it is limited to...
Whole genome variation analysis using single molecule sequencingGeneticsPharmacologyTechniques & MethodsDiscovering the genetic factors associated with common diseases and drug response has proved a daunting challenge. Genotyping has been preferred over DNA sequencing, despite its lower information content, ...
Using high-throughput genome sequencing techniques and subsequent Sanger sequencing of detected variants, we have identified ten de novo mutations in the two strains, of which six are unique to GT-O2 cells. The presence of these unique mutations highlights the need to know the genomic background ...