Circulating cell-free DNA (cfDNA) is becoming an increasingly important research sample to investigate circulating tumor DNA (ctDNA) for investigation of many cancer types. Usingwhole-genome next-generation sequencing(NGS), ...
Whole-genome sequencing is suitable for the study of disease-specific genomic abnormalities from cfDNA. TheInvitrogen Collibri PS DNA Library Prep Kit for Illumina Systemsenables consistent and reproducible cfDNA library prepar...
Whole-genome DNA sequencing 来自 ResearchGate 喜欢 0 阅读量: 64 作者: G Myers 摘要: Scitation is the online home of leading journals and conference proceedings from AIP Publishing and AIP Member Societies 关键词: ieee computer society DOI: 10.1109/5992.764214 被引量: 123 年份: 1999 ...
reference sequences sought by HGP scientists. Some genome projects were conducted without the use of such maps, using instead an approach calledwhole genome shotgun sequencing. This approach avoided the time and expense needed to create physical maps and provided more-rapid access to the DNA ...
What supplemental data is available for variant analysis in whole exome/whole genome sequencing projects? For human resequencing data, DNASTAR provides access to the Variant Annotation Database, which contains variant information using coordinates from GRCh37 (hg19) and GRCh38. Annotations… Show more...
本发明公开了一种基于克隆DNA混合池的全基因组测序方法. The present invention discloses a method for whole genome sequencing DNA-based hybrid clone pool. 该方法首先构建BAC文库并构建BAC克隆DNA混合池,对混合池DNA进行双末端的高通量测序;解析克隆的特征序列集合与k-mer集合,利用克隆的特征序列构建克隆重叠群,...
genome re-sequencing, paired-end libraries with a 400–500 bp span size were used. The fragments of template DNA from the constructed libraries were hybridized to the cell surface and then subjected to amplification to form clusters. The DNA fragments were then sequenced using an Illumina Hi...
Extraction of high molecular weight DNA and long-read whole genome sequencing All clinicalActinobacillusstrains (n = 24) and CCUG type/reference strains (n = 6) were grown and subjected to DNA extraction. All colonies from a fresh overnight culture were resuspended in 250 μL dPBS ...
Whole-genome sequencing and prediction of susceptibility For WGS of isolates, we prepared the DNA using the cetyltrimethylammonium bromide extraction method of DNA purification [15]. Genomic DNA was then sequenced using the Illumina Hiseq 2000 platform. Paired-end reads were mapped with Stampy [16...
Whole Exome Sequencing (WES) uses Next Generation Sequencing methodology to provide targeted sequence information from the coding regions of the genome.