What is the promoter sequence, and why is it important for gene activity? What is the function of the primers in a PCR reaction? What is reverse transcriptase, and why is it an important tool of recombinant DNA technology? Describe the steps of how a PCR generates m...
The RNA promoter provides its function by providing a binding site for RNA polymerase I, the enzyme that transcribes rRNA, as well as associated transcription factors. The promoter sequence on the DNA is different than most genes, which use a thymine and adenine series of nucleotides called a ...
What is the function of the trpR terminator? Operon: Operon may be defined as a group of adjacent genes that function in a harmonized manner as a genetic unit. Its components, namely, a promoter, an operator and a structural gene are involved in the transcription process as one unit. ...
Promoter elements are short sequences of DNA that bind to a cell's transcriptional initiation factors. Prokaryotes have three promoter elements: one that is upstream of the gene being transcribed, one that is 10 nucleotides downstream of it and one that is 35 nucleotides downstream. Eukaryotes hav...
What is the lagging strand in DNA replication? What do single-stranded binding proteins do in DNA replication? Which process requires DNA to undergo duplication before it can proceed? What is a promoter in DNA replication? What structure forms as DNA unwinds?
It has been shown that high salt conditions, reduced negative supercoiling and the formation of complex nucleoprotein structures in a promoter region can contribute to or even generate σS selectivity. Here, we characterize the first positively acting σS-selective feature in the promoter sequence ...
A consensus sequence for SmcR binding has been described in a previous report (JBC 283:23610). Is there a similar binding site in hlyU promoter? What is the relationship between SmcR binding site and -35/-10 of hlyU promoter? C3. 解釋的方式清晰,前後一致,不矛盾。 Data shown in Figure ...
In the seedy underground of illegal prizefighting, a corrupt boxing promoter is embroiled in a dangerous fight-fixing scheme with his female prizefighter. Arena (2011) David Lord finds himself forced into the savage world of a modern gladiatorial arena, where men fight to the death for the ente...
Thepromoter, a DNA sequence that liesupstreamof the RNA coding region, serves as an indicator of where and in which direction transcription should proceed. The promoter is not actually transcribed; its role is purely regulatory. While promoters vary tremendously among eukaryotes, there are some comm...
Codons and Anticodons A codon is a triplet sequence of RNA bases that code for a specific amino acid. The anticodon contains complementary bases to the codon. This allows the anticodon to base pair with the codon. Each anticodon has a specific amino acid attached to it....