coli involves any of the 16 possible dinucleotide primers and depends on promoter sequences in, upstream, and downstream of the primer binding site. The results yield a consensus sequence for primer-dependent initiation, Y TSS2 N TSS1 N TSS W TSS+1 , where TSS is the transcription start ...
Nucleotides flanking the promoter sequence influence the transcription of the yeast mitochondrial gene coding for ATPase subunit 9. The conserved nonanucleotide sequence functions as a promoter in the yeast (Saccharomyces cerevisiae) mitochondrial genome. A mitochondrial gene, Oli 1, wh... Biswas,T.,...
but it is possible that additional unknown sequence elements as well as the physicochemical properties of promoter DNA contribute to promoter strength35,36. Likewise, our understanding of transcription regulation is limited to factors modulating the recruitment of PICs37,38where ...
Promoter-Specific activation of RNA polymerase II transcription by SP1 The RNA polymerase II transcription factor Sp l is a protein that binds to specific D NA sequences and activates RNA synthesis from a select group of promo... JT Kadonaga,KA Jones,R Tjian - 《Trends in Biochemical ...
1d,e). Thus, by coupling Micro-C with nascent RNA-seq, we can more precisely delineate which chromatin loops are associated with active transcription in a cell type of interest. We note that Micro-C assay is superior to Hi-C at detecting E–P/P–P contacts (Extended Data Fig. 1h)26...
DNA SEQUENCE WITH TRANSCRIPTION PROMOTER ACTIVITY IN YEASTFLEER, REINHARDFALCONE, CLAUDIOSALIOLA, MICHELE
A minimal promoter region contained within the 212 bp immediately upstream of the major transcription start site contained no consensus sequences for the binding of known transcription factors, but gel mobility shift assays showed this region to bind nuclear factors, including Sp1, at a number of ...
in length(GenBank accession EF222286).Promoter sequence analyzed by PLACE and PlantCARE showed that it had TATA-box,CAAT-box and some cis-acting element such as MYB binding site,cis-acting regulatory element involved in ABA response,some light-induced responsive elements and other transcription ...
Utilization of all four initiation sites increased in response to gamma-interferon (IFN-gamma). Transient transfection analysis was used to examine cis-acting sequence motifs controlling transcription from the 5'-flanking region. We identified a 228-bp minimal promoter fragment which was able to ...
By subcloning successively smaller DNA fragments into a β-galactosidase expression vector plasmid, we located the promoter within a sequence bounded by a TaqI site at +14 with respect to the transcription startpoint and a HpaII site at 208. Measurements in vivo of β-galactosidase expression and...