However, general DNase I sensitivity and DNA methylation patterns were found to be very similar in the mutated cells and in the parental cells. These findings suggest that the promoter and active transcription play a relatively limited role in maintaining transcriptionally potentiated epigenetic states....
Role of a GC-rich motif in transcription regulation of the adenovirus type 2 IVa2 promoter which lacks typical TATA-box element Promoter of the adenovirus type 2 IVa2 (IVa2) gene does not have a TATA-box element, hence it is considered to be a model of cellular TATA-less promoters. ....
DNA methylation has been suggested to play a role in maintaining the stability of cell differentiation and in regulating gene transcription [17, 18]. It has been recently reported that the selective demethylation of a promoter DNA may be a general mechanism required for the reprogramming of somatic...
The IVa2 protein has roles in both transcription and encapsidation but the relative importance of the two functions to virus viability has not been determined. In an attempt to clarify this, a virus genome in which the IVa2 transcription binding sites (DE1 and DE2) were comprehensively mutated...
1d,e). Thus, by coupling Micro-C with nascent RNA-seq, we can more precisely delineate which chromatin loops are associated with active transcription in a cell type of interest. We note that Micro-C assay is superior to Hi-C at detecting E–P/P–P contacts (Extended Data Fig. 1h)26...
TNFalpha induces expression of transcription factors c-fos, Egr-1, and Ets-1 in vascular lesions through extracellular signal-regulated kinases 1/2. factors Ets-1, Egr-1 and c-fos in different types of vascular lesions, their regulation by TNFα and the role of ERK 1/2 in these signaling...
SQUAMOSA promoter binding protein-like (SPL) family plays vital regulatory roles in plant growth and development. The SPL family in climacteric fruit Carica papaya has not been reported. This study identified 14 papaya SPLs (CpSPL) from papaya genome and analyzed their sequence features, phylogeny,...
www.nature.com/scientificreports OPEN Critical Role of Transcription Factor PU.1 in the Function of the OX40L/ TNFSF4 Promoter in Dendritic Cells received: 24 March 2016 accepted: 19 September 2016 Published: 06 October 2016 Takuya Yashiro1,2,3, Mutsuko Hara2, Hideoki Ogawa2, Ko ...
The control of transcription initiation at the lactose operon promoter was investigated in vitro. We found that an upstream promoter (termed lac P2) interfered with RNA polymerase binding at the principal promoter (termed lac P1). The st... TP Malan,WR Mcclure - 《Cell》 被引量: 229发表: ...
Protein-DNA interactions show that SoxS and Rob, like MarA, bind the same marbox in the promoter. Electrophoretic mobility shift analyses with a MarA-specific antibody demonstrate that MarA and RNA polymerase form a ternary complex with the promoter DNA. Transcription experiments and potassium ...