Promoter and regulatory sequences of END1, a pea gene that is specifically expressed in anthers. With the present invention we characterize the pea END1 gene promoter that is capable of directing anther specific expression in early stages of its development. On the other hand, its use is ...
Sequence features of de novo promoters and enhancers To identify sequence determinants of human promoter activity, we assayed the activity of the binary STARR-seq library consisting of random sequences placed in the position of both the promoter and the enhancer (Fig. 1a, iv). For this analysis...
Archaeal viruses lacking Aca8 homologs have diverse regulatory sequences associated with their putative ADGs (Fig.1D, E and2). Although the core promoter, composed of the TATA-box and the BRE element, is highly similar among the consensus sequences, the upstream and downstream regions vary sign...
2m). Notably, an RNA model trained on expression levels of TFs, obtained from the Catalog of Inferred Sequence Binding Preferences database, only scored only 63.8% on prediction accuracy (Fig. 2m). The significantly lower predictive performance of TF expression compared to TF activity could be ...
(HMMs) on the histone modification data for known promoters and enhancers, and then used the trained HMMs to identify promoter or enhancer like sequences in the human genome. Using a simulated annealing (SA) procedure, we searched for the most informative combination and the optimal window size...
In this report, 1) we performed an in silico analysis of the bovine genome to further characterize the PAG gene family, 2) we scrutinized proximal promoter sequences of the PAG genes to evaluate the evolution pressures operating on them and to identify putative regulatory regions, 3) we ...
promotersequences MagaliLescot 1,2 ,PatriceDéhais 1 ,GertThijs 2 ,KathleenMarchal 2 ,YvesMoreau 2 , YvesVandePeer 1 ,PierreRouzé 1,3 andStephaneRombauts 1, * 1 VakgroepMoleculaireGenetica,DepartementPlantengenetica,VlaamsInteruniversitairInstituutvoor Biotechnologie,UniversiteitGent,K.L.Ledeganckstr...
1,2,3,4,5 Among these methods, it is particularly effective to increase protein expression by optimization of vector components, such as promoter, Kozak sequence and signal domain, enhancer element, poly(A), intron and splice sites, internal ribosome entry sites, codon optimization, and marker ...
Table S4. Sequences of gRNA oligos and ssODNs used to generate the CRISPR mutants, related to Figures 4 and 5 Table S5. Metadata for the Mef2 and tin , bap , and bin datasets, including quality metrics, cluster membership, annotations, and UMAP coordinates, related to Figures 4 and 5Show...
Cloned libraries for KO, KD and control sgRNAs (non-targeting and safe-targeting) were sequence validated as previously described (https://portals.broadinstitute.org/gpp/public/dir/download?dirpath=protocols/production&filename=cloning_of_oligos_for_sgRNA_shRNA_nov2019.pdf). Viral libraries were...