The present invention is related to a novel promoter which is derived from a Staphylococcus aureus phage and is functional in both Gram-positive and Gram-negative microorganisms. More specifically, isolated nucleic acid molecules comprising the promoter are provided, as are vectors and host cells ...
这里举例的这种质粒载体DNA是经过人工改造的,包含其他的DNA组件比如表达抗生素的基因序列、启动子序列、多克隆位点(multiple cloning site, MCS)等等,并且,其可以和其他一些经过基因工程改造的辅助质粒(helper plasmid)一起在工程细胞(比如293T细胞)...
Expression of a β-globin gene is enhanced by remote SV40 DNA sequences. Cell 27, 299–308 (1981). Article CAS PubMed Google Scholar Banerji, J., Olson, L. & Schaffner, W. A lymphocyte-specific cellular enhancer is located downstream of the joining region in immunoglobulin heavy chain ...
•••启动子:RNA聚合酶特异性识别和结合的DNA序列。promoter自然不属于intron和Exon的任何一个，属于noncodingsequence。noncodingRNA是现在研究的热点之一。我们常见的MiRNA,SiRNA,antisenseRNAtech,这些都是属于ncRNA的范围。只要你在进一步问下：这些RNA是哪里来的？你就知道部分答案，跟那些看似跟编码蛋白没有关系...
The prostate gland is the most common site of cancer and the second leading cause of cancer death in American men. Recent emerging molecular biological technologies help us to know that epigenetic alterations such as DNA methylation within the regulatory
第三部分 运用STS查找已经公布的引物序列STS,序列标签位点(Sequence Tagged Site ): 一段短的 DNA 序列( 200 - 500 个碱 基对),这种序列在染色体上只出现一次,其位置和碱基 36、顺序都是已知的。在PCR反应中可以检测处STS来,STS适宜于作为人类基因组的一种地标,据此可以判定DNA的方向和特定序列的相对位置。
Standard PCR or quantitative real-time PCR are often used to measure the amount of enrichment of a particular DNA sequence by a protein-specific immunoprecipitation (1,2). Alternatively, the ChIP assay can be combined with genomic tiling micro-array (ChIP on chip) techniques, high throughput ...
Archaeal promoters seem to comprise fewer promoter elements compared to their bacterial and eukaryotic counterparts, but it is possible that additional unknown sequence elements as well as the physicochemical properties of promoter DNA contribute to promoter strength35,36. Likewise, our understanding of tr...
If cells detach 48 to 72 hours post-transfection: - If the cells lift off in large sheets, this may be a sign of lentivirus production. Sequences provided for our vectors have been compiled from information in sequence databases, published sequences, and other sources....
1、彻底搞清楚promoter, exon, intron, and UTR启动子: RNA聚合酶特异性识别和结合的DNA序列。promoter自然不属于intron和Exon的任何一个,属于noncoding sequence。noncoding RNA是现在研究的热点之一。我们常见的MiRNA,SiRNA, antisense RNA tech,这些都是属于ncRNA的范围。只要你在进一步问下:这些RNA是哪里来的?你就...