“what is pKa?” and “what is pI” without ignominy. A Crash Course on Logarithms The “p” in pH, pKa, and pI denotes the negative logarithm, to base 10, of the parameter in question. Logarithms transform a nonlinear series of values into a linear series. E.g., the logarithm...
You have a 50X stock of buffer and you need 1 50 mu l of 1x. How do you make this? Which will have the lowest pKa? Why? a. H2O b. H2Se c. H2S What is the pka of KHCrO_4? How would you go about increasing the buffering capacity of a phosphate buffer?
The reducing power of DTT is affected by pH value, and it can only exert its reducing effect when the pH value is greater than 7. This is because only the deprotonated thiolate anion (-S–) is reactive, but thiol (-SH) is not; and the PKa of the thiol group is generally ~8.3. B...
What is the pI of arginine assuming the alpha-amino and guanidinium functional groups have a pKa = 10, and the carboxyl pKa = 4? What is the enzyme inhibited by your assigned inhibitor (antimycin A)? Explain the purpose of the enzyme trypsin. ...
We have identified a lysine residue (Lys-359) in the polymerase active site that protonates the pyrophosphate (PPi) leaving group during nucleotidyl transfer. Because both Lys-359 and PPi have pKa value in the 9-10 regime, proton transfer between these molecules should be maximized. However,...
Chemistry vocabulary and Chemistry glossary are different from the normal vocabulary. It is a branch of natural science that examines the building blocks of matter, including the atoms, molecules, and ions that make up compounds and their composition, structure, behaviour, and changes that occur ...
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Hormone transporters are crucial for plant hormone action, which is underlined by severe developmental and physiological impacts caused by their loss-of-function mutations. Here, we summarize recent knowledge on the individual roles of plant hormone tran
Which of the following solutions is the most acidic? (a) 0.2 m LiOH (b) 0.2 m HI What is strong about a strong acid. What is common to all acids? What forms of glutamic acid will be present at a pH of 4.3? (pKa = 2.2, 4.3, and 9.7) ...
Given that lysine has the following pKa values: pK1 = 2.2; pK2 =9.2; pK3 = 10.8 What is the pI of lysine? What does the position of each peak (where along the horizontal axis the peak is positioned) in a photoelectron spectrum tell you?