B. Large shifts in pKa values of lysine residues buried inside a protein. Proc. Natl Acad. Sci. USA 108, 5260-5265 (2011).Isom DG, Castaneda CA, Cannon BR, Garcia-Moreno E. B. Large shifts in pK(a) values of lysine residues buried inside a protein. Proc. Natl. Acad. Sci. U. ...
2015, Physiological Reviews Large shifts in pKa values of lysine residues buried inside a protein 2011, Proceedings of the National Academy of Sciences of the United States of America pK values of the ionizable groups of proteins 2006, Protein Science View all citing articles on Scopus ...
Reductive methylation of lysine residues in proteins offers a way to introduce13C methyl groups into otherwise unlabeled molecules. The13C methyl groups on lysines possess favorable relaxation properties that allow highly sensitive NMR signal detection. One of the major limitations in the use of reduc...
Lys-66 and Glu-66, buried in the hydrophobic interior of staphylococcal nuclease by mutagenesis, titrate with pK values of 5.7 and 8.8, respectively (Dwyer et al., 2000, values when the protein interior was treated with a dielectric constant () of 10. This high apparent polarizability can be...
contributes to cisplatin sensitivity in lung cancer cells via regulation of the ERK pathway, especially in cancer cells with mutated SET domain containing 2 (SETD2), a histone H3 lysine 36 (H3K36) trimethyltransferase [99]. Except for CREB, the cAMP/PKA-regulated phosphoprotein DARPP-32 ...
The majority of pKa values in protein unfolded states are close to the amino acid model pKa values, thus reflecting the weak intramolecular interactions present in the unfolded ensemble of most proteins. We have carried out thermal denaturation measurements on the WT and eight mutants of HEWL from...
Carbon-13 NMR studies of the lysine side chains of calmodulin and its proteolytic fragments ThepKa values measured for calcium saturated calmodulin ranged between 9.5 (Lys 75) and 10.2 (Lys 13); two residues (Lys 94 and Lys 13) showed a ... M.,Entazul,Huque,... - 《Journal of Protein...
The measured values are in a similar range as previously reported for the PKAC–PKI interaction in the presence of other ATP analogues or ADP71. Full size image ARHGAP36 inhibits PKAC catalytic activity As ARHGAP36 and PKAR share the same binding site on PKAC, we next investigated whether ...
Right panel: corresponding histograms of the prebleach (red) and postbleach (blue) Venus-YFP lifetime values together with the donor only control sample (black). demonstrate that ARHGAP36 is a pseudosubstrate inhibitor of PKAC with potency comparable to PKI. ARHGAP36 causes depletion of PKAC....
An accurate density functional theory based estimation of pK(a) values of polar residues combined with experimental data: from amino acids to minimal prote... Shigeta. "An accurate density functional theory based estimation of pKa values of polar residues combined with experimental data: from amino...