Answer the following questions regarding PCR reactions: \\ a. What do dNTPs provide during DNA replication? How are they different from dNMPs?\\ b. Why is Taq DNA polymerase commonly used in PCR, as opposed to, for example, mammalian DNA polymerase?\\ c. ...
What two things does a DNA polymerase require before it can do its work? 1. PCR is a tool in the molecular geneticist toolbox that allow scientists to? 2. What are the primers and what are their function? What are the three things required for DNA replication? How does this c...
Because all of the assays are amplified in the same tube, they compete for the same reagents (dNTPs, Mg2+, and polymerase). The more targets that are assayed in a multiplex reaction, the more likely it is that there will be competition for reagents and ...
The real time PCR instrument used for performing amplification must be capable of distinguishing precisely between these fluorescent labels and measuring the signals produced by the amplification of each gene. Typically, the probe for your gene of interest is labeled with ...
PCR reactions were performed in a total volume of 25 [micro]L, including 1.5 mM MgCl2, 50 mM KCl, 10 mM Tris-HCl (pH 9.0), 0.1% Triton X-100, 200 [micro]M dNTPs each (Fermentas, Germany), 25 pmoL of each primer, 1.5 U of Taq DNA polymerase (Fermentas, Germany), and 3 [micr...
The master mix must be well mixed and then split by the number of reactions, ensuring that each reaction will contain the same amount of enzyme, dNTPs, and primers. Many suppliers, such as Enzo Life Sciences, also offer PCR mixes that already contain everything except primers and the DNA...
Bridge PCR refers to the fact that during the annealing step, the extension product from one primer forms a bridge to the other bound primer and its complementary strand is produced. This sequencing techniques uses reversible terminators bound to dNTPs in a cyclical method that comprises of ...
1. Helicases: conserved but versatile players in biological processes utilizing nucleic acids in viruses and hosts 2. The structural basis for helicase translocation, directionality, and unwinding 3. The nidovirus helicase: a multi-functional enzyme controlling key steps in viral replication 4. Nidoviru...
What are the steps in DNA replication? How DNA replication occurs? What do dNTPs provide during DNA replication? How are they different from dNMPs? Explain the process of DNA replication. Explain the process of replication. Why does this process produce identical DNA strands?
(c) The use of dideoxynucleotides. (d) The use of Taq polymerase. (e) a and c. Why are ddNTPs used in Sanger sequencing, and how do they differ functionally from dNTPs? Do ddNTPs differ from d...