2.2.2 Components of PCR A conventional PCR reaction mix consists of target DNA, two primers, heat-stable DNA polymerase, deoxynucleotide triphosphates (dNTPs including dATP, dCTP, dGTP and dTTP), and a buffer usually containing Mg2+. Primers are short (20– 30 base pairs) oligonucleotides of...
to dNDPs to regenerate the dNTPs consumed by nucleo- side diphosphokinase (38), and thus help increase the DNA yield. The pWGA system performs fast isothermal DNA amplification without the need of thermocycling, prior heat-denaturation, or added primers. In this system, separation of DNA ...
To compare the performance of the PRI-lock system in the Biotrove OpenArray™ with the performance in conventional real-time PCR, samples containing single or multiple targets were ligated and analyzed on the Biotrove OpenArray™ platform. The obtained CT values were found to be similar to ...
The DNA was amplified by FIREpolDNA polymerase FIREPol® DNA Polymerase (Solis BioDyne, Tartu, Estonia) in a 25 μL solution containing 1× buffer B, 0.2 mM dNTPs, 2.5 mM MgCl2, 1 pmol/μL of each primer, 2 μL of DNA, and 0.1 U/μL of enzyme. Optionally, the 1× S ...
The PCR mixture (Taq PCR kit, New England Biolabs, Ispwich, MA, USA), contained the PCR buffer (10 mM Tris-HCl pH 8.3, 50 mM KCl with 1.5 mM MgCl2 included), 0.2 mM of dNTPs and 0.4 µM of each primers with E6-HPV16-pUC57 synthetic plasmid using as a template. The DNA ...