Figure 2. Measurement of enzyme fidelity by a PCR-based forward mutation assay. Enzyme fidelity was measured by amplifying the lacIQgene and assessing its function in E. coli. A mutation introduced during PCR in the lacIQ gene will resul... BM Slater,S Selman,B Mogilevsky,... 被引量: ...
本PCR试剂盒带有YT Taq DNA Polymerase(YT373)、PCR buffer、dNTP和上样缓冲液,自备模板和引物即可进行PCR反应,适合用于各种常规PCR以及T载体克隆、点突变等,能在高效扩增DNA模板的情况下同时保证高保真性。本试剂盒用于50微升的PCR反应体系,足够用于160个反应,用于20微升的PCR反应体系,足够用于400个反应。
Fig. 2: Rate of mutagenesis as a function of the number of PCR-cycles [Zaccoloet al.] For general laboratory use. Shipping:shipped on gel packs Storage Conditions:store at -20 °C avoid freeze/thaw cycles, aliquoting of the modified nucleotides prior to use is strongly recommen...
have found that dNTP quality and purity can directly affect dynamic range, sensitivity and precision of qPCR assays. Surprisingly, dNTP preparations that fail our qPCR functional testing are often >99% triphosphate purity by HPLC analysis and function perfectly well in conventional end-point PCR ...
Difference between NTP, dNTP and ddNTP: NTP: The key molecules that function as a source of energy in all of the cellular events linked with the...Become a member and unlock all Study Answers Start today. Try it now Create an account Ask a question Our experts can answer your tough...
Procedures to introduce point mutations into specific DNA fragments are important tools to study gene function (2,10). UV light and chemical muta-gens have been used in vivo to increase the frequency of random mutagenesis for specific DNA targets carried on plasmid or phage vector...
Which of the following enzymes play an important role in DNA replication? (a) DNA helicase (b) DNA ligase (c) DNA primase (d) All of them (e) DNA polymerase III. The main function of DNA po...
Dut is ubiquitous and essential in most investigated cases10,11,12,13,14,15,16. Recently, novel functions of Dut emerged in gene expression regulation as well15,17,18,19,20. Our genetic experiments also suggested that the mycobacterial Dut has a yet unknown but essential moonlighting function...
Such inactivity causes accumulation of dCTP and lack of dTTP, which is not available for its twofold function as DNA precursor and allosteric stimulator of GDP reduction. TK1, albeit limiting in rMt, has a crucial role in dTTP synthesis, as demonstrated by the reduction in DNA replica- tion ...
elevated levels (10 µM) of H215A–SAMHD1 were employed in the assay, confirming its critical function in catalysis. Moreover, the D218A substitution, although not entirely abolishing activity, heavily reduced the apparentkcatby ~40-fold (Fig.6a, b), consistent with its stabilising ...